R4268

Sigma-Aldrich

RNA Sample Loading Buffer

for NA electrophoresis, with ethidium bromide (50 μg/mL)

MDL number:
NACRES:
NA.25
Pricing and availability is not currently available.

Quality Level

grade

for molecular biology

form

liquid

concentration

1.25 ×

application(s)

electrophoresis: suitable

foreign activity

RNase, none detected

storage temp.

−20°C
−20°C

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General description

RNA loading buffer is used as a tracking dye during RNA electrophoresis. The RNA loading dye has a slight negative charge and will migrate the same direction as RNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition. Dilute 1:3 to 1:6 with sample, heat to 65C for ten minutes and chill on ice before loading.

Application

RNA sample loading buffer is especially formulated for electrophoresis of RNA on formaldehyde-agarose gels with or without ethidium bromide. Ethidium bromide is not recommended for gel staining prior to Northern blot detection because the presence of ethidium bromide in agarose gels or the loading buffer can cause poor transfer efficiency.
Suitable for use with formaldehyde-agarose gels used in Northern blotting procedures.
RNA Sample Loading Buffer has been used as a sample loading buffer in northern blot.

Components

Deionized formamide 62.5% (v/v), formaldehyde 1.14 M, bromphenol blue 200 μg/mL, xylene cyanole 200 μg/mL, MOPS-EDTA-sodium acetate at 1.25× working concentration.
RNA loading buffer contains 62.5% deionized formamide, 1.14M formaldehyde, 200 μg/ml bromphenol blue, 200 μg/ml xylene cyanole, and 50 μg/ml ehtidium bromide in MOPS-EDTA-sodium acetate at 1.25x working concentration.

Quantity

Recommended usage: Add 1 volume sample to 2-5 volumes of sample loading buffer and mix well. The sample should be heated to 65 °C for 10 minutes, and then chilled on ice immediately before loading on the gel.

Pictograms

Exclamation markHealth hazard

Signal Word

Danger

Target Organs

Blood

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Identification and characterization of bicistronic speB and prsA gene expression in the group A Streptococcus.
Ma Y, et al.
Journal of Bacteriology, 188, 7626-7634 (2006)
Amy E Bryant et al.
Journal of medical microbiology, 68(3), 456-466 (2019-01-25)
Extracellular protein toxins contribute to the pathogenesis of Staphylococcus aureus infections. The present study compared the effects of iclaprim and trimethoprim - two folic acid synthesis inhibitors - with nafcillin and vancomycin on production of Panton-Valentine leukocidin (PVL), alpha haemolysin...
Dennis L Stevens et al.
The Journal of infectious diseases, 195(2), 202-211 (2006-12-28)
Extracellular protein toxins contribute to the pathogenesis of a wide variety of Staphylococcus aureus infections. The present study investigated the effects that cell-wall active antibiotics and protein-synthesis inhibitors have on transcription and translation of genes for Panton-Valentine leukocidin, alpha-hemolysin, and...
The effect of massive small bowel resection and oral epidermal growth factor therapy on SGLT-1 distribution in rabbit distal remnant.
Chung BM, et al.
Pediatric Research, 55, 19-26 (2004)
Impact of antibiotics on expression of virulence-associated exotoxin genes in methicillin-sensitive and methicillin-resistant Staphylococcus aureus.
Stevens DL, et al.
The Journal of Infectious Diseases, 195, 202-211 (2007)

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