R6895

Sigma-Aldrich

Transcript RNA Markers 0.1-1 kb

buffered aqueous solution

Pricing and availability is not currently available.

grade

for molecular biology

form

buffered aqueous solution

usage

sufficient for 25 uses

shipped in

dry ice

storage temp.

−70°C

Application

Suitable for size determination of small RNA using native or denaturing agarose gel electrophoresis or PAGE gel electrophoresis. The recommended agarose gel concentration is 1.25%. A sample of the marker should be diluted with gel loading buffer (R 4268) to an appropriate loading concentration. Typically, 2 μg per well is sufficient to be seen with ethidium bromide staining.

Other Notes

Contains seven defined RNA transcripts, 100–1000 nucleotides

Physical form

Solution in 10 mM Tris-HCl, pH 8.0, 1 mM EDTA

RIDADR

NONH for all modes of transport

WGK Germany

3

Kouki Shioya et al.
PloS one, 6(9), e23948-e23948 (2011-09-14)
Small RNA molecules (sRNAs) are key mediators of virulence and stress inducible gene expressions in some pathogens. In this work we identify sRNAs in the gram positive opportunistic pathogen Enterococcus faecalis. We characterized 11 sRNAs by tiling microarray analysis, 5'...

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