R7647

Sigma-Aldrich

Random Nonamers

for use as primers in cDNA synthesis, 50 μM in H2O

NACRES:
NA.31
Pricing and availability is not currently available.

Quality Level

grade

for molecular biology

form

liquid

usage

0.1 mL sufficient for 100 RT-PCR reactions

concentration

50 μM in H2O

shipped in

wet ice

storage temp.

−20°C

General description

Random Nonamers are random sequences of nine deoxyribonucleotides (9-mers) used to prime mRNA, with or without a poly(A) tail. Random nonamers may be used as universal, non-specific primers.

Packaging

100 μL in poly bottle

Application

Random Nonamers are random sequences of nine deoxyribonucleotides (9-mers). Random nonamers may be used as universal primers in first strand cDNA synthesis, cDNA library construction, RT-PCR and other applications.
Suitable for:
  • First strand cDNA synthesis
  • Preparing cDNA libraries (with oligo (dT) primers
  • Prepare labeled DNA probes for hybridizations

Components

Random Nonamers are provided as a 50 uM solution in water.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

P Goelet et al.
Proceedings of the National Academy of Sciences of the United States of America, 79(19), 5818-5822 (1982-10-01)
Oligonucleotide primers have been used to generate a cDNA library covering the entire tobacco mosaic virus (TMV) RNA sequence. Analysis of these clones has enabled us to complete the viral RNA sequence and to study its variability within a viral...
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 10-10 (1989)
Hsiao-Wen Lin et al.
Journal of neurochemistry, 111(3), 808-818 (2009-08-29)
Interleukin-6 (IL-6) is produced by neurons, astrocytes, and microglia, and elevated levels of IL-6 within the CNS have been documented in multiple neurological disorders including Alzheimer's disease, stroke, epilepsy, attention deficit disorder, cerebral palsy, and multiple sclerosis. Here, we sought...
E M Brooks et al.
BioTechniques, 19(5), 806-812 (1995-11-01)
The secondary structure in mRNA is essential for many processes, but it can present a technical problem in making full-length cDNA with reverse transcriptases. Furthermore, different reverse transcriptases have differing abilities to transcribe through regions with secondary structure, which can...
Ondrej Hajdusek et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(4), 1033-1038 (2009-01-28)
Ticks are among the most important vectors of a wide range of human and animal diseases. During blood feeding, ticks are exposed to an enormous amount of free iron that must be appropriately used and detoxified. However, the mechanism of...

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