P Goelet et al.
Proceedings of the National Academy of Sciences of the United States of America, 79(19), 5818-5822 (1982-10-01)
Oligonucleotide primers have been used to generate a cDNA library covering the entire tobacco mosaic virus (TMV) RNA sequence. Analysis of these clones has enabled us to complete the viral RNA sequence and to study its variability within a viral...
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 10-10 (1989)
Hsiao-Wen Lin et al.
Journal of neurochemistry, 111(3), 808-818 (2009-08-29)
Interleukin-6 (IL-6) is produced by neurons, astrocytes, and microglia, and elevated levels of IL-6 within the CNS have been documented in multiple neurological disorders including Alzheimer's disease, stroke, epilepsy, attention deficit disorder, cerebral palsy, and multiple sclerosis. Here, we sought...
E M Brooks et al.
BioTechniques, 19(5), 806-812 (1995-11-01)
The secondary structure in mRNA is essential for many processes, but it can present a technical problem in making full-length cDNA with reverse transcriptases. Furthermore, different reverse transcriptases have differing abilities to transcribe through regions with secondary structure, which can...
Ondrej Hajdusek et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(4), 1033-1038 (2009-01-28)
Ticks are among the most important vectors of a wide range of human and animal diseases. During blood feeding, ticks are exposed to an enormous amount of free iron that must be appropriately used and detoxified. However, the mechanism of...