All Photos(1)

R9782

Sigma-Aldrich

RS-1

≥98% (HPLC)

Synonym(s):
4-Bromo-N-(4-bromophenyl)-3-[[(phenylmethyl)amino]sulfonyl]-benzamide, RAD51-stimulatory compound 1, 3-[(benzylamino)sulfonyl]-4-bromo-N-(4-bromophenyl)benzamide
Empirical Formula (Hill Notation):
C20H16Br2N2O3S
CAS Number:
Molecular Weight:
524.23
MDL number:
PubChem Substance ID:
NACRES:
NA.77

Quality Level

assay

≥98% (HPLC)

form

powder

color

off-white to light tan

solubility

DMSO: ≥10 mg/mL

storage temp.

room temp

SMILES string

Brc1ccc(NC(=O)c2ccc(Br)c(c2)S(=O)(=O)NCc3ccccc3)cc1

InChI

1S/C20H16Br2N2O3S/c21-16-7-9-17(10-8-16)24-20(25)15-6-11-18(22)19(12-15)28(26,27)23-13-14-4-2-1-3-5-14/h1-12,23H,13H2,(H,24,25)

InChI key

SWKAVEUTKGKHSR-UHFFFAOYSA-N

Application

RS-1 has been shown to enhance CRISPR genome editing efficiency. To see other small molecule CRISPR enhancers, visit sigma.com/CRISPR-enhancers.
RS-1 has been used:
  • as a homology-directed repair (HDR) agonist to study its effects on in human hematopoietic stem/progenitor cells (HSPCs)
  • as HDR agonist to analyze its effects on DNA repair modulation in human induced pluripotent stem (iPS) cells
  • as RAD51 agonist to study its effects on double-stranded break repair

Packaging

5, 25 mg in glass bottle

Biochem/physiol Actions

RS-1 is a sulfonamido-benzamide compound.
RS-1 (RAD51-stimulatory compound 1) is a stimulator of the human homologous recombination (HR) protein RAD51. RS-1 stimulates binding of hRAD51 to single stranded DNA (ssDNA) and enhances recombinogenic activity by stabilizing the active form of hRAD51 filaments without inhibiting hRAD51 ATPase activity. RS-1 has been shown to enhance CRISPR-Cas9 knock-in efficiency in HEK293A cells and has been shown to enhance both TALEN and Cas9-mediated knock-in efficiency in rabbits.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Gemma Moir-Meyer et al.
Methods and protocols, 1(3) (2019-06-06)
The study of cellular processes and gene regulation in terminal erythroid development has been greatly facilitated by the generation of an immortalised erythroid cell line derived from Human Umbilical Derived Erythroid Precursors, termed HUDEP-2 cells. The ability to efficiently genome...
Liqian Zhu et al.
Veterinary research, 48(1), 45-45 (2017-09-09)
Bovine herpesvirus 1 (BoHV-1) infection enhanced the generation of inflammatory mediator reactive oxidative species (ROS) and stimulated MAPK signaling that are highly possibly related to virus induced inflammation. In this study, for the first time we show that BoHV-1 infection...
Xue Wang et al.
BioMed research international, 2019, 7878906-7878906 (2019-11-07)
It has been reported that paclitaxel administration could cause sensorineural hearing loss, and Wnt activation is important for the development and cell protection of mouse cochlea. However, the effect of Wnt signaling in spiral ganglion neurons (SGNs) damage induced by...
Siti Nur Syahirah Ahmad et al.
Foods (Basel, Switzerland), 10(2) (2021-01-31)
This study aims to investigate the effect of different vegetable oils and frying cycles on acrylamide formation during the intermittent frying of beef nuggets. Different vegetable oils, palm olein (PO), red palm olein (RPO), sunflower oil (SFO), and soybean oil...
Jun Song et al.
Nature communications, 7, 10548-10548 (2016-01-29)
Zinc-finger nuclease, transcription activator-like effector nuclease and CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR-associated protein 9) are becoming major tools for genome editing. Importantly, knock-in in several non-rodent species has been finally achieved thanks to these customizable nucleases; yet...

Articles

Small Molecule CRISPR Enhancers

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