Sena Bluemel et al.
Gut microbes, 11(3), 265-275 (2019-04-16)
Alcohol-induced liver disease is closely related to translocation of bacterial products and bacteria from the intestine to the liver. However, it is not known whether bacterial translocation to the liver depends on certain intestinal microbiota changes that would predispose bacteria...
Tomas Erban et al.
Scientific reports, 7(1), 5084-5084 (2017-07-13)
Honeybee (Apis mellifera L.) workers act as passive vectors of Paenibacillus larvae spores, which cause the quarantine disease American foulbrood (AFB). We assessed the relative proportions of P. larvae within the honeybee microbiome using metabarcoding analysis of the 16 S rRNA...
Tong Ihn Lee et al.
Nature protocols, 1(2), 729-748 (2007-04-05)
Genome-wide location analysis, also known as ChIP-Chip, combines chromatin immunoprecipitation and DNA microarray analysis to identify protein-DNA interactions that occur in living cells. Protein-DNA interactions are captured in vivo by chemical crosslinking. Cell lysis, DNA fragmentation and immunoaffinity purification of...
Sambrook, J., and Russell, D.W.
Molecular Cloning: A Laboratory Manual, A1-A1 (2001)
Nadin Rohland et al.
Nature protocols, 13(11), 2447-2461 (2018-10-17)
DNA preserved in ancient bones, teeth and sediments is typically highly fragmented and present only in minute amounts. Here, we provide a highly versatile silica-based DNA extraction protocol that enables the retrieval of short (≥35 bp) or even ultrashort (≥25...