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T0256

Sigma-Aldrich

Trypsin inhibitor from bovine pancreas

Type I-P, essentially salt-free, lyophilized powder

Synonym(s):
BPTI
CAS Number:
EC Number:
MDL number:

biological source

bovine pancreas

type

Type I-P

form

essentially salt-free, lyophilized powder

mol wt

~6500 Da (single-chain 58-amino acid peptide)

purified by

crystallization

technique(s)

inhibition assay: suitable

solubility

water: 10 g/L

storage temp.

−20°C

Packaging

1, 5, 10, 25 mg in glass bottle

Biochem/physiol Actions

A 58-residue protein whose binding to various serine proteases has been extensively studied. It is highly homologous to a family of mamba snake dendrotoxin proteins that inhibit various K+ channels. It also binds to an intracellular site associated with the large conductance Ca2+-activated K+ channel..

Unit Definition

One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.

Preparation Note

Prepared by method of Kunitz and Northrup, J. Gen. Physiol., 19, 991 (1936).

Analysis Note

One mg of trypsin inhibitor will inhibit greater than 1.5 mg of trypsin with activity of approximately 10,000 BAEE units per mg protein.
Protein determined by biuret.

Other Notes

View more information on Trypsin Inhibitor.

Certificate of Analysis

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Certificate of Origin

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Technical Information

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