T9039

Sigma-Aldrich

Tris Buffered Saline, with Tween® 20, pH 8.0

powder

Synonym(s):
TBST

form

powder

Quality Level

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Related Categories

Application

Tris Buffered Saline, with Tween® 20, pH 8.0 has been used as a wash buffer in western blotting and enzyme linked immunosorbent assay. It has also been used along with 5% milk to block proteins on nitrocellulose in western blotting.

Biochem/physiol Actions

Tris Buffered Saline with Tween 20 is predominantly used as a wash buffer and also in blocking steps of immunoblotting technique.

Packaging

Foil pouches

Reconstitution

Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.05 M Tris buffered saline (NaCl - 0.138 M; KCl - 0.0027 M); TWEEN® 20 - 0.05%, pH 8.0, at 25 °C.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Joey P Ting et al.
PloS one, 13(1), e0190850-e0190850 (2018-01-13)
To date, IL-17A antibodies remain the only therapeutic approach to correct the abnormal activation of the IL-17A/IL-17R signaling complex. Why is it that despite the remarkable success of IL-17 antibodies, there is no small molecule antagonist of IL-17A in the...
Differential antibody responses to gliadin-derived indigestible peptides in patients with schizophrenia
McLean RT, et al.
Translational Psychiatry, 7(5), e1121-e1121 (2017)
Matrix cells from Wharton's jelly form neurons and glia
Mitchell KE, et al.
Stem Cells, 21(1), 50-60 (2003)
Isabell Rall et al.
Viruses, 11(12) (2019-12-11)
Parrot bornaviruses (PaBVs) are the causative agents of proventricular dilatation disease (PDD), a chronic and often fatal neurologic disorder in Psittaciformes. The disease is widely distributed in private parrot collections and threatens breeding populations of endangered species. Thus, immunoprophylaxis strategies...
Study of circulating IgG antibodies to BIRC5 and MYC in non-small cell lung cancer
Ye Leiguang, et al.
FEBS Open Bio, 5(1), 809-812 (2015)
Protocols
Cultrex™ 3-D Culture Cell Harvesting Kit provides an optimized and standardized solution for the isolation and normalization of cell lysates from 3-D Culture Matrix™ BME or Laminin I for subsequent biochemical analysis.
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