Documents
W1754
Water
PCR Reagent
Recommended Products
![Water, PCR Grade liquid, pkg of 25 mL (03315959001 [1 x 25 ml]), pkg of 25 mL (03315932001 [25 x 1 ml]), pkg of 100 mL (03315843001 [4 x 25 ml])](/deepweb/assets/sigmaaldrich/product/images/326/208/bac83eed-3c88-4738-b4b5-50d3b223dd1c/640/bac83eed-3c88-4738-b4b5-50d3b223dd1c.jpg)
grade
PCR Reagent
Quality Level
vapor density
<1 (vs air)
vapor pressure
3 mmHg
sterility
sterile-filtered
form
liquid
packaging
vial of 1.5 mL
technique(s)
PCR: suitable
refractive index
n20/D 1.34 (lit.)
pH
5-7
bp
100 °C (lit.)
mp
0 °C (lit.)
density
1.000 g/mL at 3.98 °C (lit.)
foreign activity
DNase, none detected
RNase, none detected
SMILES string
O
InChI
1S/H2O/h1H2
InChI key
XLYOFNOQVPJJNP-UHFFFAOYSA-N
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Related Categories
1 of 4
This Item | W4502 | W3513 | 34877 |
|---|---|---|---|
| vapor density <1 (vs air) | vapor density <1 (vs air) | vapor density <1 (vs air) | vapor density <1 (vs air) |
| vapor pressure 3 mmHg | vapor pressure 3 mmHg | vapor pressure 3 mmHg | vapor pressure 3 mmHg |
| form liquid | form liquid | form liquid | form liquid |
| technique(s) PCR: suitable | technique(s) PCR: suitable, electrophoresis: suitable | technique(s) cell culture | mammalian: suitable | technique(s) HPLC: suitable |
| refractive index n20/D 1.34 (lit.) | refractive index n20/D 1.34 (lit.) | refractive index n20/D 1.34 (lit.) | refractive index n20/D 1.34 (lit.) |
General description
Application
- as a component of the reaction mixture and as a diluting agent in microfluidic RT-qPCR
- as a component of the reaction mixture for the amplification of products from fungal (Trametes versicolor) DNA
- as a diluting agent and as a component of the reaction mixture for the amplification of cDNA
Suitability
Other Notes
Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
No data available
Flash Point(C)
No data available
Personal Protective Equipment
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Protocols
Protocol using hot start dNTPs. Method includes modified nucleoside triphosphates that block DNA polymerase nucleotide incorporation during hot start PCR to increase specificity. Compatible with a variety of PCR reagents.
Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
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