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A0157

Ascorbate Oxidase from Cucurbita sp.

lyophilized powder, 1,000-3,000 units/mg protein

Synonym(s):

L-Ascorbate:oxygen oxidoreductase, Ascorbase

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About This Item

CAS Number:
9029-44-1
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-852-6
MDL number:
MFCD00130560
EC Number:
1.10.3.3 (BRENDA, IUBMB)

Key Documents

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Product Name

Ascorbate Oxidase from Cucurbita sp., lyophilized powder, 1,000-3,000 units/mg protein

biological source

plant (Cucurbita spp.)

form

lyophilized powder

specific activity

1,000-3,000 units/mg protein

storage temp.

2-8°C

Quality Level

200

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This Item
189724P410510736619001
Ascorbate Oxidase from Cucurbita sp. lyophilized powder, 1,000-3,000 units/mg protein

Sigma-Aldrich

A0157

Ascorbate Oxidase from Cucurbita sp.

Ascorbate Oxidase, Cucurbita sp. Native ascorbate oxidase from Cucurbita species. Catalyzes the oxidation of ascorbioxidation of ascorbic acid to dehydroascorbic acid. Suitable for eliminating interference of ascorbic acid in clinical assays and for determination of ascorbic acid.

Sigma-Aldrich

189724

Ascorbate Oxidase, Cucurbita sp.

Pyruvate Oxidase from Aerococcus sp. lyophilized powder, ≥35 units/mg protein (biuret)

Sigma-Aldrich

P4105

Pyruvate Oxidase from Aerococcus sp.

Ascorbate Oxidase Spatula from Cucurbita species

Roche

10736619001

Ascorbate Oxidase Spatula

specific activity

1,000-3,000 units/mg protein

specific activity

≥1500 U/mg, ≥300 units/mg material

specific activity

≥35 units/mg protein (biuret)

specific activity

17 units

biological source

plant (Cucurbita spp.)

biological source

-

biological source

bacterial (Aerococcus spp.)

biological source

plant (Cucurbita species)

form

lyophilized powder

form

lyophilized

form

lyophilized powder

form

solid

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

100

Application

Ascorbate Oxidase from Cucurbita sp. has been used:
  • as a supplement in the culture medium for differentiation into osteoblasts[1]
  • to oxidize ascorbic acid producing monodehydroascorbate in monodehydroascorbate reductase assay[2]
  • in determining ascorbate (AsA) and dehydroascorbate (DHA) concentrations[3]

Ascorbate oxidase, from Cucurbita sp., may be used to study oxidative stress and heat stress response and tolerance. Ascorbate oxidase, from Sigma, has been used in ascorbic acid assays to study the heat stress response of Arabidopsis [4].

Biochem/physiol Actions

Ascorbate oxidase (AO) participates in cell growth by regulating reduction/oxidation (redox) of the apoplast. This enzyme can synthesize the oxidative molecule dehydroascorbate acid (DHA) in the apoplast. It is also involved in cell elongation and enlargement development.[3]
Ascorbate oxidase converts ascorbic acid to dehydroascorbic acid. It is highly specific for L-ascorbic acid and a few analogs. Ascorbate oxidase exists as a dimer and has a molecular weight of approximately 140 kDa.

General description

Ascorbate oxidase is a homodimeric enzyme, which comprises 552 amino acid residues per subunit (zucchini). It corresponds to a molecular mass of 70kDa per subunit.[5] This enzyme is mainly found in plants, fungi and eubacteria.[6]

Other Notes

One unit will oxidize 1.0 μmole of L-ascorbate to dehydroascorbate per min at pH 5.6 at 25 °C.

Physical form

Lyophilized powder containing buffers and sucrose as stabilizer.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000465064
0000465062
0000465060
0000465060
0000465062

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Michael Wawire et al.
Journal of agricultural and food chemistry, 59(5), 1774-1783 (2011-02-12)
Cowpea, an African leafy vegetable ( Vigna unguiculata ), contains a high level of vitamin C. The leaves harvested at 4-9 weeks are highly prone to vitamin C losses during handling and processing. Therefore, the purpose of this research was
Ann Wambui Munyaka et al.
Journal of food science, 75(4), C336-C340 (2010-06-16)
The thermal stability of vitamin C (including l-ascorbic acid [l-AA] and dehydroascorbic acid [DHAA]) in crushed broccoli was evaluated in the temperature range of 30 to 90 degrees C whereas that of ascorbic acid oxidase (AAO) was evaluated in the
Kyoko Fujita et al.
Biopolymers, 93(12), 1093-1099 (2010-07-29)
Hydrated choline dihydrogen phosphate (Hy[ch][dhp]) containing 30 wt% water was investigated as a novel protein solvent. The Hy[ch][dhp] dissolved some metallo proteins (cytochrome c, peroxidase, ascorbate oxidase, azurin, pseudoazurin and fructose dehydrogenase) without any modification. These proteins retained the surroundings
Nidhi Chauhan et al.
The Analyst, 136(9), 1938-1945 (2011-03-19)
An ascorbate oxidase (AsOx) (E.C.1.10.3.3) purified from Lagenaria siceraria fruit was immobilized covalently onto a carboxylated multiwalled carbon nanotubes and polyaniline (c-MWCNT/PANI) layer electrochemically deposited on the surface of an Au electrode. The diffusion coefficient of ascorbic acid was determined
Ethylene response factor 6 is a regulator of reactive oxygen species signaling in Arabidopsis
Sewelam N, et al.
PLoS ONE, 8(8), e70289-e70289 (2013)
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