All Photos(2)

A0185

Sigma-Aldrich

Monoclonal Anti-Biotin−Peroxidase antibody produced in mouse

clone BN-34, purified immunoglobulin

Synonym(s):
Monoclonal Anti-Biotin
NACRES:
NA.46

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

BN-34, monoclonal

packaging

vial of 0.5 mL

application(s)

dot blot: suitable
flow cytometry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300
indirect ELISA: 1:30,000
western blot (chemiluminescent): 1:80,000

isotype

IgG1

conjugate

peroxidase conjugate

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

General description

The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immuno­histo­chemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. Specificity is evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates.
Monoclonal Anti-Biotin (mouse IgG1 isotype) has been derived from the BN-34 hybridoma produced by the used successfully for detection of micro-injected, fusion of mouse myeloma cells and splenocytes from biotin-haptenized cytoskeletal proteins which enable BALB/c mice immunized with biotinylated keyhole limpet hemocyanin (KLH).

Immunogen

Biotin-KLH conjugate

Application

Monoclonal Anti-Biotin Peroxidase antibody produced in mouse has been used:
  • in immunohistochemistry
  • in western blot
  • as secondary antibody to detect the presence of aptamer on the membrane

The antibody may be used in amplification techniques, ELISA, immunoblotting, in situ nucleic acid hybridization, flow cytometry, image analysis or confocal microscopy.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.

Biochem/physiol Actions

Biotin is an essential vitamin required for cells in living organisms and in cultures. The high binding affinity of biotin to egg white avidin or bacteria-derived streptavidin has been used to design immunoassays for detecting antigen-antibody interactions. The use of monoclonal anti-biotin IgG can enhance the sensitivity of such avidin-biotin immunoassays. The monoclonal antibody functions by selectively enlarging and bridging the avidin-biotin-enzyme complexes, thereby increasing the signals from substrate conversion. Furthermore, this antibody can be used in many other applications where biotin may be introduced as target label.
Monoclonal Anti-Biotin-Peroxidase antibody specific for biotin conjugated to various proteins. The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immuno­histo­chemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. The product specificity has been evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates.

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

SIX1 is overexpressed in endometrial carcinoma and promotes the malignant behavior of cancer cells through ERK and AKT signaling
Xin X, et al.
Oncology Letters, 12(5), 3435-3440 (2016)
Improved scaffold biocompatibility through anti-Fibronectin aptamer functionalization
Galli C, et al.
Acta Biomaterialia, 42, 147-156 (2016)
Yinan Wei et al.
The Journal of biological chemistry, 281(33), 23492-23502 (2006-06-23)
YiiP is a representative member of the cation diffusion facilitator (CDF) family, a class of ubiquitous metal transporters that play an essential role in metal homeostasis. Recently, a pair of Zn2+/Cd2+-selective binding sites has been localized to two highly conserved...
Alexander Postel et al.
Analytical biochemistry, 411(1), 22-31 (2010-12-22)
There is an urgent need for robust subtype-specific serological tests to diagnose influenza A virus infections in poultry and mammals, including humans. Such assays require reliable subtype-specific sources of soluble and authentically folded seroreactive hemagglutinin (HA), one of the integral...
Monica Sueiro-Olivares et al.
PLoS biology, 19(6), e3001247-e3001247 (2021-06-02)
Aspergillus fumigatus is a human fungal pathogen that can cause devastating pulmonary infections, termed "aspergilloses," in individuals suffering immune imbalances or underlying lung conditions. As rapid adaptation to stress is crucial for the outcome of the host-pathogen interplay, here we...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service