A0293

Sigma-Aldrich

Anti-Human IgG (Fab specific)−Peroxidase antibody produced in goat

affinity isolated antibody

Synonym(s):
Anti Human Fab Antibody, Anti Human Fab Antibody - Anti-Human IgG (Fab specific)-Peroxidase antibody produced in goat
MDL number:
NACRES:
NA.46

Quality Level

biological source

goat

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

human

application(s)

direct ELISA: 1:40,000
dot blot: 1:100,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
western blot (chemiluminescent): 1:100,000

conjugate

peroxidase conjugate

shipped in

dry ice

storage temp.

−20°C

Related Categories

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders,.
Antibody is isolated from anti-human IgG antiserum by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fab fragment of human IgG. Anti-Human IgG is conjugated to peroxidase and then further purified to remove unconjugated material.

Immunogen

Fab fragment of human IgG

Application

Anti-Human IgG (Fab specific)-Peroxidase antibody produced in goat has been used in:
  • western blot
  • direct enzyme linked immunosorbent assay (ELISA) at 1:40,000 dilution for analysis of yeast cultures genetically modified to produced recombinant Fab fragments.
  • immunohistochemistry
  • dot blot
  • immunohistochemistry (formalin-fixed, paraffin-embedded sections, 1:200)

Anti-Human IgG antibody was used at a 1:10,000 dilution in PBS and incubated for 1 hour at room temperature. Binding in ELISA assays was detected with a O-phenylene-diamine substrate solution (Sigma).
Goat anti-human IgG (Fab specific)-peroxidase antibody can be used for direct ELISA (1:40,000), dot blot and immunohistochemistry (formalin-fixed, paraffin-embedded sections, 1:200) applications.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Christopher Tipper et al.
Journal of virology, 87(7), 3628-3639 (2013-01-25)
After entering a host cell, retroviruses such as simian immunodeficiency virus (SIV) uncoat, disassembling the viral capsid. Rates of uncoating that are too high and too low can be detrimental to the efficiency of infection. Rapid uncoating typically leads to...
James Duehr et al.
mSphere, 3(1) (2018-02-13)
Recent reports in the scientific literature have suggested that anti-dengue virus (DENV) and anti-West Nile virus (WNV) immunity exacerbates Zika virus (ZIKV) pathogenesis in vitro and in vivo in mouse models. Large populations of immune individuals exist for a related...
Deng Ning et al.
Journal of biochemistry, 134(6), 813-817 (2004-02-11)
Anti-HBs Fab fragment has considerable potential for use in the prevention and treatment of liver diseases by HBV. Here we established a high-level expression system to directly produce anti-HBs Fab fragment in Pichia pastoris. This was achieved by co-integration of...
Kristell Lebozec et al.
mAbs, 9(6), 945-958 (2017-06-10)
Glycoprotein VI is a platelet-specific collagen receptor critical for in vivo formation of arterial thrombosis. It is also considered as an attractive target for the development of anti-thrombotic drugs because blocking glycoprotein (GP)VI inhibits platelet aggregation without inducing detrimental effects...
A novel approach of prophylaxis to HBV recurrence after liver transplantation
Pan T, et al.
Virology, 382(1), 1-9 (2008)
Articles
Antibody-based serology tests are useful in identifying subjects with an adaptive immune response to the SARS-CoV-2 virus. Anti-human immunoglobulin antibodies allow for quick and simple, yet reliable assays with easy readouts and can also be adapted for high-throughput screening.
Read More

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