A0349

Anti-l-Afadin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-AF6, Anti-MLL-AF6, Anti-MLLT4, Anti-l-afadin
• MDL number: MFCD03455360
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~200 kDa
• species reactivity: canine, mouse, rat, human
• packaging: antibody small pack of 25 μL
• enhanced validation: independent; Learn more about Antibody Enhanced Validation
• technique(s): immunohistochemistry (frozen sections): 1:1,000 using mouse liver sections.; indirect immunofluorescence: 1:500 using MDCK cells and cultured human HepG2 cells.; microarray: suitable; western blot: 1:2,000 using extract of rat brain
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... MLLT4(4301) ; mouse ... Mllt4(17356) ; rat ... Mllt4(26955)

Description

General description

Afadin is a multidomain protein that binds F-actin and E-catenin proteins at cell-cell adheren junctions. I-afadin is a ubiquitously expressed splice variant of afadin that contains PDZ and F-actin binding domains at the C-terminal end. I-afadin is homologous to the human AF-6 protein .

Specificity

Anti-l-Afadin antibody is specific for I-afadin (approximately 200 kDa). It also binds I-afadin in rat, mouse, dog and human.

Immunogen

Synthetic peptide sequence corresponding to amino acid residues 1814-1829 of rat afadin with N-terminal cysteine conjugated to maleimide-activated KLH.

Application

Anti-l-Afadin antibody produced in rabbit has been used in:

• western blotting
• immunofluorescence experiments
• immunocytochemistry
• immunohistochemistry

Biochem/physiol Actions

AF-6/Afadin regulates the formation of tight and adherens junctions in the cell and its absence in mice leads to embryonic defects and lethality . Afadin is implicated in primary breast cancer and pancreatic cancer. It downregulates the process of migration and metastasis in cancer cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable