A0409

Anti-α₁-Antitrypsin antibody produced in rabbit

IgG fraction of antiserum

• Synonym(s): Alpha 1 Antitrypsin Antibody, Alpha 1 Antitrypsin Antibody - Anti-α1-Antitrypsin antibody produced in rabbit, anti-A1AT
• MDL number: MFCD00162392
• NACRES: NA.46

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• mol wt: antigen ~50 kDa
• species reactivity: human
• packaging: vial of 2 mL lyophilized antiserum
• technique(s): immunoelectrophoresis: suitable; indirect ELISA: 1:30,000-1:60,000
• UniProt accession no.: P01009
• storage temp.: 2-8°C
• Gene Information: human ... SERPINA1(5265)

Description

General description

α-1-antitrypsin belongs to the serpin family and is a strong serine protease inhibitor. Its pivotal role is to protect lower respiratory tract against proteolytic destruction by human leukocyte elastase. Mutation in AAT gene will reduce the serum concentration of α-1-antitrypsin and hence increase the risk of emphysema. Anti-α1-antitrypsin antibody (diluted 1:9000) can be used as a capture antibody for determination of transgene expressions levels. It can also be used in immunoelectrophoresis. Rabbit anti-α1-antitrypsin antibody reacts specifically with α1-antitrypsin of human.

Immunogen

Purified human α-1-antitrypsin

Application

Anti-α1-antitrypsin antibody (diluted 1: 1000) can be used in ELISA. It can also be used for probing immunoblots to identify A1AT. Additionally, it can be used in Ouchterlony double diffusion.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Rabbit anti-α1-antitrypsin antibody can also be used in immunoelectrophoretic techniques.

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.2.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable