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A1031

Sigma-Aldrich

α-Amylase from human saliva

greener alternative

Type XIII-A, lyophilized powder, 300-1,500 units/mg protein

Synonym(s):

1,4-α-D-Glucan-glucanohydrolase

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1000 UNITS
$170.00
5000 UNITS
$596.00

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1000 UNITS
$170.00
5000 UNITS
$596.00

About This Item

CAS Number:
EC Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54

$170.00


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type

Type XIII-A

Quality Level

form

lyophilized powder

specific activity

300-1,500 units/mg protein

composition

Protein, 5-15% biuret

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Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

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UniProt accession no.

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storage temp.

−20°C

Gene Information

human ... AMY1A(276)

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This Item
A0521A638010069
Gene Information

human ... AMY1A(276)

Gene Information

human ... AMY1A(276)

Gene Information

-

Gene Information

-

specific activity

300-1,500 units/mg protein

specific activity

1,000-3,000 units/mg protein

specific activity

≥1,500 units/mg protein (biuret)

specific activity

~380 U/mg

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

UniProt accession no.

P0DUB6

UniProt accession no.

P0DUB6

UniProt accession no.

-

UniProt accession no.

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

General description

Human α-amylase is made up of 496 amino acids in a single polypeptide chain, which is encrypted on chromosome 1. These are produced either in the salivary glands or the pancreas. The salivary and pancreatic α-amylases have homologous primary sequence but exhibit different cleavage patterns.[1]
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Application

α-Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. Product A1031 is from human saliva, is type IIA, and is supplied as a lyophilized powder. α-Amylase has been used in various plant studies, such as metabolism studies in Arabidopsis [2]. α-Amylase from human saliva has been used to study the development of nutraceuticals, which may aid the treatment of diabetes and obesity [3].

Biochem/physiol Actions

α-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose.[4]

Unit Definition

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.

Physical form

Lyophilized powder containing (NH4)2SO4, sodium citrate and sodium chloride.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Certificates of Analysis (COA)

Lot/Batch Number

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Questions

1–5 of 5 Questions  
  1. Considering the wide range of activity range, how can I be sure of the enzyme activity of the specific batch that I receive?

    1 answer
    1. The protein content and enzymatic activity are determined on a lot to lot basis. Each value is reported in the specific Certificate of Analysis. The protein content range is 5 - 15% and the enzymatic activity range is 300 - 1500 units per milligram protein. A review of recent lots indicates typical protein concentrations of 7 - 13% and activities of 1350 - 1450 units per milligram protein. Please see the link below to review a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/a1031#product-documentation

      Helpful?

  2. ¿Cómo se reconstituye la enzima al venir liofilizada? ¿Qué tasa de actividad tiene?

    1 answer
    1. For assay purposes, this enzyme is reconstituted in water at 1 u/mL. This material is also soluble in 1 mM Calcium Chloride. The activity specification is 300 - 1500 U/mg. The unit per milligram value is reported in the lot-specific Certificate of Analysis and will range from 0.67 to 3.3 mg for the 1000 U pack size. Please see the link below to review a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/a1031#product-documentation

      Helpful?

  3. Hi, could you let me know what the remaining composition is when the product contains 5-15% protein? Thank you!

    1 answer
    1. As mentioned in the 'DESCRIPTION' section, this product is a lyophilized powder containing (NH4)2SO4, sodium citrate and sodium chloride. This product is not tested for purity. However, buffer salts are expected to be the bulk of the non-protein content.

      Helpful?

  4. Please let me know the storage temperature and storage period when it is dissolved in water

    1 answer
    1. The stability of this material in solution has not been investigated. However, various sources suggest that solutions may be stored in working aliquots at -20°C for several months.

      Helpful?

  5. What is the recommended method for dissolving alpha amylase obtained from human saliva, specifically A1031?

    1 answer
    1. The recommended method is to dissolve the enzyme in 1mM calcium chloride (CaCl2).

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