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MilliporeSigma

A1227

ABTS Enhancer

liquid, for peroxidase detection

Synonym(s):

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Enhancer

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.83

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Product Name

ABTS Enhancer, for peroxidase detection

Quality Level

100

form

liquid

storage temp.

2-8°C

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This Item
11557A9941A1888
ABTS Enhancer for peroxidase detection

Sigma-Aldrich

A1227

ABTS Enhancer

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt ≥98% (HPLC)

Sigma-Aldrich

11557

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt tablet, 10 mg substrate per tablet

Sigma-Aldrich

A9941

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt ≥98% (HPLC)

Sigma-Aldrich

A1888

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

form

liquid

form

powder or crystals

form

tablet

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

room temp

storage temp.

2-8°C

Application

2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) has been used for the determination of laccase activity in Trametes versicolor.[1] It may be suitable for use in enzyme-linked immunosorbent assay (ELISA) for detecting signal strength emitted by cells.[2]

Biochem/physiol Actions

2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) is a non-phenolic substrate.[3] It might exhibit antioxidant property.[4] The detection of the horseradish peroxidase substrate ABTS can be improved by the addition of a chemical enhancer. The ABTS enhancer increases the absorbance of the oxidized product 50-150%. This increase is used to improve assay sensitivity or conserve assay components.

Physical form

Supplied as an 11X concentrate

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H350,H412

Hazard Classifications

Aquatic Chronic 3 - Carc. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
MKCR7362
MKCQ9085
MKCQ0943
MKCM1154
MKCL1708

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Khaled K Abu-Amero et al.
Molecular vision, 17, 2769-2775 (2011-11-09)
To evaluate total antioxidant status (TAS) in the plasma of pseudoexfoliation glaucoma (PEG) patients and to compare this level with a matching control group. Additionally, we aim to investigate the effect of the combined action of the lysyl oxidase-like 1
Manickam Naresh Kumar et al.
Bioresource technology, 244(Pt 1), 353-361 (2017-08-07)
Inhibitor mediated intensified bio-pretreatment (IMBP) technology using natural cellulase inhibitor (NCI) for maximum cellulose recovery from paddy straw was studied. Pretreatment was carried out under solid state condition. Supplementation of 8% NCI in pretreatment medium improves cellulose recovery and delignification
Mark E Levenstein et al.
Stem cells (Dayton, Ohio), 26(12), 3099-3107 (2008-09-20)
Human embryonic stem (ES) cells can be maintained in an undifferentiated state if the culture medium is first conditioned on a layer of mouse embryonic fibroblast (MEF) feeder cells. Here we show that human ES cell proliferation is coordinated by
Investigations on antioxidant potential of phenolic acids and flavonoids: the common phytochemical ingredients in plants
Singh DP, et al.
Journal of Plant Biochemistry & Physiology (2018)
Meng-Hsuan Wu et al.
Scientific reports, 9(1), 9754-9754 (2019-07-07)
Laccases that are tolerant to organic solvents are powerful bio-catalysts with broad applications in biotechnology. Most of these uses must be accomplished at high concentration of organic solvents, during which proteins undergo unfolding, thereby losing enzyme activity. Here we show

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Related Content

Data Sheet - A1227

Instructions

Questions

1–3 of 3 Questions  

  1. How can Product A1227, ABTS Enhancer, be used?

    1 answer

    1. This product is for microwell applications such as ELISA. A soluble reaction product is produced, so this is not for applications that require a precipitation reaction such as blotting or histochemical applications.

      Helpful?

  2. Why should Product A1227, ABTS Enhancer, be used?

    1 answer

    1. This enhancer may be used to increase sensitivity or to conserve assay components.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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