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A1271

Sigma-Aldrich

Adenosine 5′-monophosphate–Agarose

lyophilized powder

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MDL number:
MFCD04113722
eCl@ss:
32160414
PubChem Substance ID:
329770699
NACRES:
NA.56

form

lyophilized powder

Quality Level

200

extent of labeling

1-5 μmol per mL

matrix

cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

matrix attachment

C-8

matrix spacer

9 atoms

storage temp.

−20°C

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This Item
A2767A9396A9272
Adenosine 5′-monophosphate–Agarose lyophilized powder

Sigma-Aldrich

A1271

Adenosine 5′-monophosphate–Agarose

Adenosine 5′-triphosphate–Agarose lyophilized powder

Sigma-Aldrich

A2767

Adenosine 5′-triphosphate–Agarose

Adenosine 2′-monophosphate from yeast

Sigma-Aldrich

A9396

Adenosine 2′-monophosphate

Adenosine 3′-monophosphate from yeast

Sigma-Aldrich

A9272

Adenosine 3′-monophosphate

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

extent of labeling

1-5 μmol per mL

extent of labeling

1-5 μmol per mL

extent of labeling

-

extent of labeling

-

matrix

cross-linked 4% beaded agarose

matrix

cross-linked 4% beaded agarose

matrix

-

matrix

-

matrix activation

cyanogen bromide

matrix activation

cyanogen bromide

matrix activation

-

matrix activation

-

matrix attachment

C-8

matrix attachment

C-8

matrix attachment

-

matrix attachment

-

Application

Adenosine 5′-monophosphate Agarose (5′-AMP agarose) has been used in affinity chromatography to isolate β and gamma glutamate decarboxylase, which is important for controlling gamma-aminobutyric acid (GABA) synthesis in brain.

Physical form

Lyophilized powder stabilized with lactose

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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705578-5MG-PW

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F James et al.
The Journal of biological chemistry, 270(38), 22344-22350 (1995-09-22)
The plant enzyme S-adenosylmethionine:methionine S-methyltransferase (EC 2.1.1.12, MMT) catalyzes the synthesis of S-methylmethionine. MMT was purified 620-fold to apparent homogeneity from leaves of Wollastonia biflora. The four-step purification included fractionation with polyethylene glycol, affinity chromatography on adenosine-agarose, anion exchange chromatography
S K Lee et al.
FEBS letters, 316(3), 261-263 (1993-02-01)
The mosquito homolog of mammalian DNA polymerase epsilon, formerly known as a proliferating cell nuclear antigen (PCNA)-independent form of DNA polymerase delta, has been purified from mosquito larval extracts. The polymerase epsilon was separated from DNA polymerase alpha by chromatography
C D Murphy et al.
Applied and environmental microbiology, 67(10), 4919-4921 (2001-09-26)
Streptomyces cattleya is unusual in that it produces fluoroacetate and 4-fluorothreonine as secondary metabolites. We now report the isolation of an NAD(+)-dependent fluoroacetaldehyde dehydrogenase from S. cattleya that mediates the oxidation of fluoroacetaldehyde to fluoroacetate. This is the first enzyme
D L Martin
Cellular and molecular neurobiology, 7(3), 237-253 (1987-09-01)
1. Glutamate decarboxylase is a focal point for controlling gamma-aminobutyric acid (GABA) synthesis in brain. Several factors that appear to be important in the regulation of GABA synthesis have been identified by relating studies of purified glutamate decarboxylase to conditions
S M Pettit et al.
Clinical chemistry, 27(1), 88-93 (1981-01-01)
We present a method for preparing human liver lactate dehydrogenase (L-lactate:NAD+ oxidoreductase; EC 1.1.1.27) isoenzyme-5 by sequential ion-exchange chromatography, general-ligand (AMP analog) affinity chromatography, and preparative isoelectric focusing. The yield ws 40%, with a 493-fold purification. The final specific activity
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