Hybri-Max, γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma

4-Amino-1-(β-D-ribofuranosyl)-1,3,5-triazin-2(1H)-one, Ladakamycin
Empirical Formula (Hill Notation):
CAS Number:
Molecular Weight:
Beilstein/REAXYS Number:
EC Number:
MDL number:
PubChem Substance ID:

Quality Level

biological source

synthetic (organic)





product line



lyophilized powder


cell culture | hybridoma: suitable


endotoxin, tested


226-232 °C (dec.) (lit.)


acetic acid: water (1:1): 5 mg/mL, clear, colorless

Mode of action

DNA synthesis | interferes

antibiotic activity spectrum


storage temp.


SMILES string




InChI key


Gene Information

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General description

Chemical structure: nucleoside


5-Azacytidine may be used to induce in vitro cardiomyogenic differentiation of adult human bone marrow mesenchymal stem cells. It may also be used to study the effects of 5-Azacytidine on the immune regulatory myeloid-derived suppressor cells (MDSCs) and regulatory T cells (Tregs).
Used to study the effects of the DNA incorporated molecule on DNA methylation processes.

Biochem/physiol Actions

5-Azacytidine is a deoxycytidine analog and a demethylating agent. It acts as a potential antineoplastic agent for acute myelogenous leukemia. 5-Azacytidine activates repressed genes by inhibiting DNA methylation. 5-Azacytidine also affects protein synthesis by altering the RNA function and stability.
A potent growth inhibitor and cytotoxic agent; inhibits DNA methyltransferase, an important regulatory mechanism of gene expression, gene activation and silencing.


Reconstitute contents of vial with 10 mL sterile cell culture medium. Stock solution is sufficient to prepare 500ml medium. Final working concentration: 10 μM 5-azacytidine.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC


Exclamation markHealth hazard

Signal Word


Hazard Statements

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Certificate of Analysis

Certificate of Origin

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the concentration of Product A1287, 5-Azacytidine - Hybri-Max?

    The working concentration is 10 μM, so the 50X stock is 500 μM.

  4. How much is contained in the 1 vial of Product A1287, 5-Azacytidine - Hybri-Max?

    1 vial contains 1.2 mg.

  5. How is Product A1287, 5-Azacytidine - Hybri-Max, to be dissolved? And once dissolved, is the solution stable?

    Dissolve each 1.2 mg vial in 10 mL of culture medium. This compound is very unstable in aqueous solutions; we have found a 10% loss after just 2-3 hours at room temperature in phosphate buffer pH 7.4, even faster loss at 50°C. We generally recommend keeping solutions on ice during the course of a day. If stock solution must be kept, store them in the freezer at -20°C in single use aliquots.  

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

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DNA methyltransferase inhibitors for cancer therapy
Epigenetics, 5(3), 265-290 (2015)
5-azacytidine treatment reorganizes genomic histone modification patterns
Komashko VM and Farnham PJ
Epigenetics, 5(3), 229-240 (2010)
In vitro cardiomyogenic differentiation of adult human bone marrow mesenchymal stem cells. The role of 5-azacytidine
Antonitsis P, et al.
Interactive Cardiovascular and Thoracic Surgery, 6(5), 593-597 (2007)
Martin F Kaiser et al.
Blood, 122(2), 219-226 (2013-05-24)
Outcome in multiple myeloma is highly variable and a better understanding of the factors that influence disease biology is essential to understand and predict behavior in individual patients. In the present study, we analyzed combined genomewide DNA methylation and gene...
Agata Magdalena Wasik et al.
Scientific reports, 3, 1400-1400 (2013-03-08)
The related transcription factors SOX11, SOX4 and SOX12 (classified as the SOXC family) compete for the same target genes. SOX11 is expressed in most mantle cell lymphomas (MCL) but a small subset is, like normal lymphocytes, SOX11 negative. Here we...

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