A1812

Sigma-Aldrich

Monoclonal Anti-FITC−Alkaline Phosphatase antibody produced in mouse

clone FL-D6, purified immunoglobulin, buffered aqueous solution

Synonym(s):
Monoclonal Anti-FITC
NACRES:
NA.46
Pricing and availability is not currently available.

biological source

mouse

recombinant

expressed in mouse cell line

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

FL-D6, monoclonal

form

buffered aqueous solution

application(s)

dot blot: 1:80,000 using human IgG/FITC monoclonal anti-human IgG
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using FITC conjugate
indirect ELISA: 1:30,000

isotype

IgG1

conjugate

alkaline phosphatase conjugate

shipped in

wet ice

storage temp.

2-8°C

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General description

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques because the conjugation of FITC to proteins is relatively easy and generally does not destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Antibodies to FITC are used to identify FITC labeled proteins and as models to study the mechanism of antibody response to a well defined hapten. Monoclonal Anti-FITC (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Specificity

The antibody reacts with both free and conjugated fluorescein isothiocyanate (FITC).

Immunogen

FITC-BSA conjugate

Application

Antibodies to FITC serve as universal indicator reagents by bridging FITC with another immunohistochemical reagent such as alkaline phosphatase or horseradish peroxidase. Mouse monoclonal clone FL-D6 anti-FITC−Alkaline Phosphatase conjugate antibody may be used for the detection of FITC and as a universal indicator reagent for bridging FITC with other immunochemical reagents. It can be used in ELISA and immunofluorescent techniques.
Alkaline phosphatase-conjugated mouse anti-FITC antibody was used for immunohistochemistry on mouse spleens. Tissue sections were fixed in acetone, blocked with PBS/5% goat serum/ 0.1% Tween and incubated with a FITC-conjugated primary antibody prior to the addition of the AP-conjugated anti-FITC antibody.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 50% glycerol, 1 mM MgCl2 and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Martyn A Sharpe et al.
EBioMedicine, 2(9), 1122-1132 (2015-10-27)
The last major advance in the treatment of glioblastoma multiforme (GBM) was the introduction of temozolomide in 1999. Treatment with temozolomide following surgical debulking extends survival rate compared to radiotherapy and debulking alone. However, virtually all glioblastoma patients experience disease...
M Hollinshead et al.
The Histochemical journal, 30(8), 577-581 (1998-10-29)
A number of histochemical chromogenic substrates for alkaline phosphatase are commercially available and give reaction products with a range of colours for brightfield examination. Some of these reaction products are also fluorescent, exhibiting a wide excitation range and a broad...
Systems biology of immunity to MF59-adjuvanted versus nonadjuvanted trivalent seasonal influenza vaccines in early childhood.
Nakaya, et al.
Proceedings of the National Academy of Sciences of the USA, 113, 1853-1858 (2021)
H Noorchashm et al.
Journal of immunology (Baltimore, Md. : 1950), 163(2), 743-750 (1999-07-08)
B cell-deficient nonobese diabetic (NOD) mice are protected from the development of spontaneous autoimmune diabetes, suggesting a requisite role for Ag presentation by B lymphocytes for the activation of a diabetogenic T cell repertoire. This study specifically examines the importance...

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