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Sigma-Aldrich

Bovine Serum Albumin

chromatographically purified, New Zealand origin, low endotoxin, suitable for cell culture, pH 7, ≥98%

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Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.27

biological source

bovine

Quality Level

product line

BioReagent

assay

≥98%

form

lyophilized powder

mol wt

~66 kDa

purified by

chromatography

packaging

poly bottle of

origin

New Zealand origin

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/mg endotoxin

pH

7

solubility

water: soluble (40 mg/ml)

UniProt accession no.

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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General description

Bovine serum albumin (BSA) is a 66 kDa globular protein crucial for transporting molecules like fatty acids, drugs and hormones from the bloodstream. BSA is a drug carrier for bioactive compounds and has wide clinical applications. BSA has extensive usage in biological research including cell culture, molecular biology, protein biochemistry, and detection techniques. It has wide pharmaceutical applications due to ease of production and interaction flexibility.

Application

Bovine serum albumin has been used:
  • as a standard in bradford protein estimation method in yeast cells
  • in elispot assay of blood cells
  • as a blocking agent in immunohistochemical studies of transplant
  • as a blocking agent in western blotting of human gastric cancer cell lines

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Features and Benefits

  • Tested for use in cell culture
  • Low endotoxin

Preparation Note

Prepared by salt fractionation, ion exchange, and gel filtration chromatography.
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Multi-spectroscopic characterization of bovine serum albumin upon interaction with atomoxetine
Buddanavar AT and Nandibewoor ST
Journal of Pharmaceutical Analysis, 7(3), 148-155 (2017)
Cloning, characterization and expression of an exo-1, 3-beta-glucanase gene from the antagonistic yeast, Pichia guilliermondii strain M8 against grey mold on apples
Zhang D, et al.
Biological Control, 59(2), 284-293 (2011)
Review on albumin as drug carrier in treating different diseases and disorders
Naveen R, et al.
Pharmacia Sinica, 7(1), 11-15 (2017)
Gene regulatory network construction identified NFYA as a diffuse subtype-specific prognostic factor in gastric cancer
Cao B, et al.
International Journal of Oncology, 53(5), 1857-1868 (2018)
Quantification of self-recognition in multiple sclerosis by single-cell analysis of cytokine production
Pelfrey CM, et al.
Journal of Immunology, 165(3), 1641-1651 (2000)

Articles

Explore serum albumin's role in serum-free cell culture systems for biomanufacturing.

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