Anti-Rabbit IgG (γ-chain specific)–Peroxidase antibody, Mouse monoclonal

clone RG-96, purified from hybridoma cell culture

Monoclonal Anti-Rabbit IgG (γ-chain specific)

Quality Level

biological source


antibody form

purified immunoglobulin

antibody product type

secondary antibodies


RG-96, monoclonal


lyophilized powder

species reactivity


should not react with

chicken, guinea pig, goat, horse, sheep, rat, bovine, human, pig, feline, canine


vial of 0.5 mL


direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
western blot: 1:160,000 using total cell extract of HeLa cells (5-10 μg/well)




peroxidase conjugate

storage temp.


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General description

The product recognizes an epitope located on the γ (heavy)-chain of rabbit IgG. In immunoblotting, the antibody recognizes both native and denatured forms of rabbit IgG. In ELISA, the antibody is specific for rabbit IgG, and shows no cross-reactivity with rabbit IgA and IgM or human IgG, IgA, and IgM. No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, or sheep.
Anti-Rabbit IgG (γ-chain specific)-Peroxidase antibody, Mouse monoclonal is a monoclonal anti-rabbit IgG conjugated to horseradish peroxidase. Monoclonal antirabbit IgG is derived from the RG-96 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with rabbit IgG.
Rabbit IgGs are often used as primary antibodies in several immunological assays. Thus, polyclonal anti-rabbit IgGs are usually used as secondary antibodies in such applications. However, polyclonal antibodies to rabbit IgGs can also cross-react with IgGs from other species, especially with human IgGs, and thereby lead to a lack of specificity in test results. The use of monoclonal anti-rabbit IgG that is specific for only rabbit IgG improves the accuracy of immunological assays and thus, can be useful for various research applications.


Rabbit IgG


Monoclonal Anti-Rabbit IgG (γ-chain specific)- Peroxidase has been used in
  • various immunochemical assays like enzyme linked immunosorbent assay (ELISA)
  • westernblotting
  • immunohistology
  • dot immunobinding assay

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

NsrR: a key regulator circumventing Salmonella enterica serovar Typhimurium oxidative and nitrosative stress in vitro and in IFN-gamma-stimulated J774. 2 macrophages
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