A1949

Anti-Rabbit IgG (γ-chain specific)–Peroxidase antibody, Mouse monoclonal

clone RG-96, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-Rabbit IgG (γ-chain specific)
• NACRES: NA.46

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: peroxidase conjugate
• antibody form: purified immunoglobulin
• antibody product type: secondary antibodies
• clone: RG-96, monoclonal
• form: lyophilized powder
• species reactivity: rabbit
• should not react with: goat, feline, pig, guinea pig, rat, bovine, canine, human, horse, sheep, chicken
• packaging: vial of 0.5 mL
• technique(s): direct ELISA: 1:40,000; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200; western blot: 1:160,000 using total cell extract of HeLa cells (5-10 μg/well)
• isotype: IgG1
• storage temp.: 2-8°C

Description

General description

Anti-Rabbit IgG (γ-chain specific)-Peroxidase antibody, Mouse monoclonal is a monoclonal anti-rabbit IgG conjugated to horseradish peroxidase. Monoclonal antirabbit IgG is derived from the RG-96 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with rabbit IgG.

Rabbit IgGs are often used as primary antibodies in several immunological assays. Thus, polyclonal anti-rabbit IgGs are usually used as secondary antibodies in such applications. However, polyclonal antibodies to rabbit IgGs can also cross-react with IgGs from other species, especially with human IgGs, and thereby lead to a lack of specificity in test results. The use of monoclonal anti-rabbit IgG that is specific for only rabbit IgG improves the accuracy of immunological assays and thus, can be useful for various research applications.

The product recognizes an epitope located on the γ (heavy)-chain of rabbit IgG. In immunoblotting, the antibody recognizes both native and denatured forms of rabbit IgG. In ELISA, the antibody is specific for rabbit IgG, and shows no cross-reactivity with rabbit IgA and IgM or human IgG, IgA, and IgM. No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, or sheep.

Immunogen

Rabbit IgG

Application

Monoclonal Anti-Rabbit IgG (γ-chain specific)- Peroxidase has been used in

• various immunochemical assays like enzyme linked immunosorbent assay (ELISA)
• westernblotting
• immunohistology
• dot immunobinding assay

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317
• Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable