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A1960

Sigma-Aldrich

Aggrecan from bovine articular cartilage

lyophilized powder (from a sterile-filtered solution)

MDL number:
NACRES:
NA.75

Quality Level

biological source

bovine articular cartilage

sterility

sterile-filtered

form

lyophilized powder (from a sterile-filtered solution)

packaging

glass bottle of 1 mg

application(s)

cell culture | mammalian: suitable

impurities

salt, essentially free

color

white

solubility

H2O: 2 mg/mL

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

Related Categories

Packaging

1 mg in glass bottle

Application

Aggrecan is a critical component for cartilage structure and the function of joints. Its synthesis and degradation are being studied for their roles in cartilage deterioration during joint injury disease and aging.

Biochem/physiol Actions

This molecule produces a rigid, reversibly deformable gel that resists compression. It combines with hyaluronic acid to form very large macromolecular complexes that have an increased hydrohynamic volume and a significant increase (30-40%) in the relative viscosity of the solution.

Components

Aggrecan is a major structural proteoglycan of the cartilage extracellular matrix. It is a large proteoglycan, with a molecular weight greater than 2,500 kDa composed of approximately 100-150 glycosaminoglycan chains attached to a core protein. The majority of these glycosaminoglycan chains are chondroitin/dermatan sulfate. Aggrecan contains three globular domains, G1, G2, and G3, which are involved in aggregation and hylauronan binding, cell adhesion and chondrycte apoptosis.

Caution

Store this product at -20°C. Stored as supplied, this powder shows little decomposition in 3 years when stored properly.

Preparation Note

This product is extracted from articular cartilage, chematographically purified, dialyzed against water, and sterile-filtered prior to lyophilization. Once lyophilized, this powder is essentially salt-free. The product is soluble in water at 2 mg/mL.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Doege, K.J. et al.
Guidebook to the Extracellular Matrix and Adhesion Proteins, 17-18 (1993)
M C Bolton et al.
The Biochemical journal, 337 ( Pt 1), 77-82 (1998-12-17)
The rates of incorporation of radiolabelled leucine into aggrecan and link protein have been measured in human articular cartilage of different ages. Aggrecan and link protein were purified in the A1 fraction of CsCl gradients as a result of their
E C Arner et al.
The Journal of biological chemistry, 274(10), 6594-6601 (1999-02-26)
A method was developed for generating soluble, active "aggrecanase" in conditioned media from interleukin-1-stimulated bovine nasal cartilage cultures. Using bovine nasal cartilage conditioned media as a source of the aggrecanase enzyme, an enzymatic assay was established employing purified aggrecan monomers
T E Hardingham et al.
European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies, 32(4), 249-257 (1994-04-01)
Aggrecan, the large aggregating proteoglycan from cartilage contains chondroitin sulphate and keratan sulphate attached to a multidomain protein core. It aggregates by binding to hyaluronan and this is further stabilised by a separate globular link protein. There are two structurally
N Ishiguro et al.
Arthritis and rheumatism, 42(1), 129-136 (1999-01-27)
To determine the relationship between matrix metalloproteinases (MMPs), their inhibitors, and the turnover of matrix molecules in articular cartilage from patients with osteoarthritis (OA). Synovial fluid samples were collected from the knees of 54 patients with OA. Radiographic evaluations and

Articles

Glycosaminoglycans and Proteoglycans

There are five identified glycosaminoglycan chains (see Figure 1): Hyaluronan is not sulfated, but the other glycosaminoglycan chains contain sulfate substituents at various positions of the chain.

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

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