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A2052

Sigma-Aldrich

Anti-GABA antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Synonym(s):
Anti Gaba, Anti Gaba Antibody, Anti-Gaba, GABA Antibody - Anti-GABA antibody produced in rabbit, Gaba Antibody, Anti-γ-Aminobutyric acid
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

rat, Drosophila, wide range

packaging

antibody small pack of 25 μL

technique(s)

dot blot: 1:10,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2.5 μg/mL using rat cerebellum

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

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SAB4200721A0310MAB341
conjugate

unconjugated

conjugate

-

conjugate

unconjugated

conjugate

-

antibody form

affinity isolated antibody

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

antibody form

purified immunoglobulin

clone

polyclonal

clone

GB-69, monoclonal

clone

GB-69, monoclonal

clone

BD17, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

-

form

-

species reactivity

rat, Drosophila, wide range

species reactivity

rat, monkey, frog, mouse, human, gerbil

species reactivity

wide range

species reactivity

mouse, feline, monkey, bovine

General description

Anti-GABA is produced in rabbit using GABA-BSA as the immunogen. The antibody is isolated from antiserum by immunospecific methods of purification. Antigen specific affinity isolation removes essentially all rabbit serum proteins, including immunoglobulins which do not specifically bind to GABA.
GABA (γ-aminobutyric acid) is a multifunctional molecule found in several organisms, from prokaryotes to vertebrates. It is present in non-nervous structures, such as peripheral nervous and endocrine systems. GABA is formed following decarboxylation of glutamic acid by the enzyme glutamic acid decarboxylase (GAD).

Immunogen

γ-aminobutyric acid (GABA) conjugated to BSA.

Application

Expression of GABA in neucortical cells harvested from the brains of E19 day old rat embyros was detected by immunofluorescence using rabbit anti-GABA antibody. Triple IF staining was performed with the anti-GABA antibody and two anti-GAD antibodies. Expression of GABA was analyzed in cells isolated from the pallium of various animals including rats, mice, rabbits, guinea pigs, and lizards by immunohistochemistry. IHC was performed using rabbit anti-GABA antibody at 1:1000 diluted in a solution of 0.01M PBS ph 7.4 + 0.5% triton-x100.
Rabbit anti-GABA antibody has been used for electron microscopy analysis in rat hippocampal tissues at a dilution of 1:4000. The antibody has also been used for immunocytochemistry applications at dilutions ranging from 1:100-1:750 in Drosophila and Periplaneta americana brain cells.

Biochem/physiol Actions

GABA (γ-aminobutyric acid) is the major inhibitory neurotransmitter in the central nervous system and also functions as a neuromodulator in some peripheral tissues. It controls heart rate, blood pressure and respiratory process. GABA facilitates neuronal inhibition by interacting with GABA/benzodiazepine receptor and opening an integral chloride channel. Rabbit anti-GABA antibody shows positive binding with GABA and GABA-keyhole limpet hemocyanin (KLH) in a dot blot assay, and exhibits negative binding with bovine serum albumin (BSA).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Effect of GABA on blood pressure and blood dynamics of anesthetic rats.
Ma P, et al.
International Journal of Clinical and Experimental Medicine, 8(8), 14296-14296 (2015)
D D Wang et al.
The Journal of physiology, 550(Pt 3), 785-800 (2003-06-17)
Previous studies have reported the presence of migrating and dividing neuronal progenitors in the subventricular zone (SVZ) and rostral migratory stream (RMS) of the postnatal mammalian brain. Although the behaviour of these progenitors is thought to be influenced by local
Central gamma-aminobutyric acid involvement in blood pressure control.
Gillis R A, et al.
Federation Proceedings, 43(1), 32-38 (1984)
Ping Wang et al.
Frontiers in cellular neuroscience, 9, 188-188 (2015-06-05)
GABAA receptors mediate fast inhibitory neurotransmission in the brain. Dysfunction of these receptors is associated with various psychiatric/neurological disorders and drugs targeting this receptor are widely used therapeutic agents. Both the efficacy and plasticity of GABAA receptor-mediated neurotransmission depends on
Rachel I Wilson et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 25(40), 9069-9079 (2005-10-07)
Drosophila olfactory receptor neurons project to the antennal lobe, the insect analog of the mammalian olfactory bulb. GABAergic synaptic inhibition is thought to play a critical role in olfactory processing in the antennal lobe and olfactory bulb. However, the properties

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