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A2064

Sigma-Aldrich

Anti-Human IgG−Alkaline Phosphatase antibody, Mouse monoclonal

clone GG-5, purified from hybridoma cell culture

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Synonym(s):
Monoclonal Anti-Human IgG (γ-chain specific)
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

alkaline phosphatase conjugate

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

GG-5, monoclonal

form

buffered aqueous glycerol solution

species reactivity

human

technique(s)

direct ELISA: 1:50,000
dot blot: 1:80,000
immunohistochemistry: suitable
western blot (chemiluminescent): 1:80,000

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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This Item
A3076A2189A9544
conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

biological source

mouse

biological source

mouse

biological source

mouse

biological source

goat

technique(s)

direct ELISA: 1:50,000, immunohistochemistry: suitable, dot blot: 1:80,000, western blot (chemiluminescent): 1:80,000

technique(s)

direct ELISA: 1:6,000, dot blot: 1:1,000

technique(s)

direct ELISA: 1:35,000, dot blot: 1:20,000 using 20 ng of human IgM/dot, dot blot: 1:30,000 using 20 ng of human IgM/dot (chemiluminescence)

technique(s)

direct ELISA: 1:55,000, dot blot: 1:40,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

affinity isolated antibody

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

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General description

Binds human IgG; does not bind other human Igs.The antibody is specific for a determinant on the heavy chain of human IgG. This antibody will form antigen-antibody complexes in the liquid phase in the presence of 3% PEG 6000.
Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.
Monoclonal Anti-Human IgG (Γ-chain specific) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Application

Anti-Human IgG- Alkaline Phosphatase antibody, Mouse monoclonal has been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • western blotting
  • dot blot
  • immunohistology

Elisa assays were performed using alkaline phosphatase conjugated monoclonal mouse anti-human IgG to detect antibodies against various S. Aureus antigens in the serum from healthy individuals. The antibody was diluted 1:30000 in PBSt and incubated with samples for 2 hours at 37 degrees. Reactin was developed using p-nitrophenyl phosphate substrate (Sigma).

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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George J Broze
Blood cells, molecules & diseases, 52(2-3), 116-120 (2013-10-01)
Acquired factor X (FX) deficiency unrelated to amyloidosis is a rare disorder in which an anti-FX antibody is infrequently detected. A patient with severe bleeding due to a calcium ion-dependent anti-FX IgG antibody is described. The FX affinity purified IgG
Werner E G Müller et al.
Journal of cell science, 128(11), 2202-2207 (2015-04-25)
Polyphosphate (polyP) is a physiologically occurring polyanion that is synthesized especially in bone-forming osteoblast cells and blood platelets. We used amorphous polyP nanoparticles, complexed with Ca(2+), that have a globular size of ∼100 nm. Because polyP comprises inorganic orthophosphate units
Receptor-mediated targeting of spray-dried lipid particles coformulated with immunoglobulin and loaded with a prototype vaccine
Bot AI, et al.
Pharmaceutical Research, 18(7), 971-979 (2001)
Safety and Immunogenicity of a Pseudomonas aeruginosa Hybrid Outer Membrane Protein F-I Vaccine in Human Volunteers
Mansouri E, et al.
Infection and Immunity, 67(7), 1461-1470 (1999)
Ana Luísa Tomás et al.
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 39(11), 2205-2209 (2020-06-20)
Interest in the detection of specific anti-Pneumocystis jirovecii antibodies has emerged as less-invasive alternative diagnostic approaches. Here is presented the performance of an ELISA based on a recombinant synthetic multi-epitope kexin 1 (Kex1) antigen of P. jirovecii, previously developed. Results

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