A2064

Sigma-Aldrich

Anti-Human IgG−Alkaline Phosphatase antibody, Mouse monoclonal

clone GG-5, purified from hybridoma cell culture

Synonym(s):
Monoclonal Anti-Human IgG (γ-chain specific)
NACRES:
NA.46
Pricing and availability is not currently available.

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

GG-5, monoclonal

form

buffered aqueous glycerol solution

species reactivity

human

application(s)

direct ELISA: 1:50,000
dot blot: 1:80,000
immunohistochemistry: suitable
western blot (chemiluminescent): 1:80,000

isotype

IgG1

conjugate

alkaline phosphatase conjugate

shipped in

wet ice

storage temp.

2-8°C

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General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.
Binds human IgG; does not bind other human Igs.The antibody is specific for a determinant on the heavy chain of human IgG. This antibody will form antigen-antibody complexes in the liquid phase in the presence of 3% PEG 6000.
Monoclonal Anti-Human IgG (Γ-chain specific) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Application

Elisa assays were performed using alkaline phosphatase conjugated monoclonal mouse anti-human IgG to detect antibodies against various S. Aureus antigens in the serum from healthy individuals. The antibody was diluted 1:30000 in PBSt and incubated with samples for 2 hours at 37 degrees. Reactin was developed using p-nitrophenyl phosphate substrate (Sigma).
Anti-Human IgG- Alkaline Phosphatase antibody, Mouse monoclonal has been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • western blotting
  • dot blot
  • immunohistology

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Werner E G Müller et al.
Journal of cell science, 128(11), 2202-2207 (2015-04-25)
Polyphosphate (polyP) is a physiologically occurring polyanion that is synthesized especially in bone-forming osteoblast cells and blood platelets. We used amorphous polyP nanoparticles, complexed with Ca(2+), that have a globular size of ∼100 nm. Because polyP comprises inorganic orthophosphate units...
Receptor-mediated targeting of spray-dried lipid particles coformulated with immunoglobulin and loaded with a prototype vaccine
Bot AI, et al.
Pharmaceutical Research, 18(7), 971-979 (2001)
Safety and Immunogenicity of a Pseudomonas aeruginosa Hybrid Outer Membrane Protein F-I Vaccine in Human Volunteers
Mansouri E, et al.
Infection and Immunity, 67(7), 1461-1470 (1999)
S Thordardottir et al.
Scandinavian journal of immunology, 59(6), 574-581 (2004-06-09)
This study was conducted to investigate the frequency and origin of discrepant assay results between two haemolytic assays which both measure activity of the classical pathway of complement (CH50) by haemolysis of sheep red blood cells (SRBCs). One is conducted...
E Mansouri et al.
Infection and immunity, 67(3), 1461-1470 (1999-02-20)
A hybrid protein [Met-Ala-(His)6OprF190-342-OprI21-83] consisting of the mature outer membrane protein I (OprI) and amino acids 190 to 342 of OprF of Pseudomonas aeruginosa was expressed in Escherichia coli and purified by Ni2+ chelate-affinity chromatography. After safety and pyrogenicity evaluations...
Articles
Antibody-based serology tests are useful in identifying subjects with an adaptive immune response to the SARS-CoV-2 virus. Anti-human immunoglobulin antibodies allow for quick and simple, yet reliable assays with easy readouts and can also be adapted for high-throughput screening.
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