A2220

ANTI-FLAG^® M2 Affinity Gel

purified immunoglobulin, buffered aqueous glycerol solution

• Synonym(s): Monoclonal ANTI-FLAG^® M2 antibody produced in mouse, ANTI-FLAG^® M2 Affinity Agarose Gel, Anti-ddddk, Anti-dykddddk
• NACRES: NA.32

Properties

• conjugate: agarose conjugate
• Quality Level: 200
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: M2, monoclonal
• form: buffered aqueous glycerol solution
• analyte chemical class(es): proteins (FLAG^® tag, 3x FLAG^®, DYKDDDDK tag)
• technique(s): affinity chromatography: suitable (FLAG^® peptide, Glycine, pH3.5, 3x FLAG^® peptide); immunoprecipitation (IP): suitable
• matrix: (4% agarose bead; 45-165μm bead size)
• isotype: IgG1
• capacity: >0.6 mg/mL, resin binding capacity (FLAG-BAP)
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Anti-FLAG M2 Affinity gel is a mouse monoclonal antibody that is covalently attached to agarose. The antibody binds FLAG at the N-terminal, Met-N-terminal, C-terminal and internal locations of fusion proteins. Binding is calcium-independent.

Elution - FLAG^® peptide, Glycine, pH 3.5, 3x FLAG^® peptide

Immunogen

DYKDDDDK

Application

Anti-FLAG^® M2 affinity gel has been used for western blotting, immunoprecipitation and for the purification of FLAG fusion proteins.

Browse additional application references in our FLAG^® Literature portal.

Physical form

Suspension in buffered saline containing azide as preservative and 50% glycerol

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable