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A2220

Millipore

ANTI-FLAG® M2 Affinity Gel

purified immunoglobulin, buffered aqueous glycerol solution

Synonym(s):
Monoclonal ANTI-FLAG® M2 antibody produced in mouse, ANTI-FLAG® M2 Affinity Agarose Gel, Anti-ddddk, Anti-dykddddk
NACRES:
NA.32

conjugate

agarose conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous glycerol solution

analyte chemical class(es)

proteins (FLAG® tag, 3x FLAG®, DYKDDDDK tag)

technique(s)

affinity chromatography: suitable (FLAG® peptide, Glycine, pH3.5, 3x FLAG® peptide)
immunoprecipitation (IP): suitable

matrix

(4% agarose bead; 45-165μm bead size)

isotype

IgG1

capacity

>0.6 mg/mL, resin binding capacity (FLAG-BAP)

shipped in

wet ice

storage temp.

−20°C

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This Item
M8823SAB420007111988506001
conjugate

agarose conjugate

conjugate

magnetic beads

conjugate

unconjugated

conjugate

fluorescein conjugate

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified from hybridoma cell culture, purified immunoglobulin

antibody form

purified immunoglobulin

clone

M2, monoclonal

clone

M2, monoclonal

clone

6F7, monoclonal

clone

3F10, monoclonal

form

buffered aqueous glycerol solution

form

suspension

form

buffered aqueous solution

form

lyophilized

technique(s)

affinity chromatography: suitable (FLAG® peptide, Glycine, pH3.5, 3x FLAG® peptide), immunoprecipitation (IP): suitable

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable

technique(s)

immunoprecipitation (IP): 2.5-5.0 μg using lysates of transiently transfected cells expressing C-terminal-FLAG-tagged protein, western blot: 0.5-1.0 μg/mL using extracts of transiently transfected cells expressing C-terminal-FLAG-tagged protein

technique(s)

-

isotype

IgG1

isotype

IgG1

isotype

IgG1

isotype

IgG1

General description

Anti-FLAG M2 Affinity gel is a mouse monoclonal antibody that is covalently attached to agarose. The antibody binds FLAG at the N-terminal, Met-N-terminal, C-terminal and internal locations of fusion proteins. Binding is calcium-independent.

Elution - FLAG® peptide, Glycine, pH 3.5, 3x FLAG® peptide

Immunogen

DYKDDDDK

Application

Anti-FLAG® M2 affinity gel has been used for western blotting, immunoprecipitation and for the purification of FLAG fusion proteins.

Browse additional application references in our FLAG® Literature portal.

Physical form

Suspension in buffered saline containing azide as preservative and 50% glycerol

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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