A2237
Phosphatase, Alkaline shrimp
≥900 DEA units/mL, buffered aqueous glycerol solution, recombinant, expressed in proprietary host
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Synonym(s):
Phosphatase, Alkaline from shrimp, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
recombinant
expressed in proprietary host
Quality Level
form
buffered aqueous glycerol solution
concentration
≥900 DEA units/mL
foreign activity
RNAse, DNAse, none detected
shipped in
dry ice
storage temp.
−20°C
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General description
Shrimp alkaline phosphatase is a heat labile, hydrolase enzyme. It is a high specific activity alkaline phosphatase purified from a recombinant source. There are two preferred sites for enzyme activity: 5′ protruding, recessive and blunt 5′-termini. The enzyme is irreversibly heat inactivated at 65°C for 15 minutes.
Application
Alkaline phosphatase was used to examine its role in the prevention of high-fat-diet-induced metabolic syndrome in mice. It also may be used in pasteurization process for milks used in dairy products.
Biochem/physiol Actions
Alkaline phosphatase is commonly responsible for dephosphorylation of nucleotides, proteins and alkaloids. The enzyme nonspecifically catalyzes the dephosphorylation of 5′-termini DNA and RNA phosphomonoesters reactions but it does not affect diphosphate or triphosphate linkages.
Unit Definition
One unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C.
Physical form
solution in 50% glycerol containing 25 mM Tris-HCl, pH 7.5, 1 mM MgCl2
Analysis Note
Protein determined by biuret
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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