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A2289

Sigma-Aldrich

Albumin, Tetramethylrhodamine isothiocyanate bovine

lyophilized powder

Synonym(s):
TRITC-albumin
CAS Number:
MDL number:
NACRES:
NA.25

biological source

bovine

Assay

≥90% (Lowry)

form

lyophilized powder

extent of labeling

≥0.5 mol TRITC per mol albumin

technique(s)

cell culture | mammalian: suitable

impurities

salt, free

UniProt accession no.

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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A8426A4612N8159
Bovine Serum Albumin lyophilized powder, Essentially fatty acid free

Sigma-Aldrich

A4612

Bovine Serum Albumin

biological source

bovine

biological source

bovine

biological source

bovine

biological source

bovine

assay

≥90% (Lowry)

assay

≥90% protein basis (biuret)

assay

≥98% (agarose gel electrophoresis)

assay

-

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

ELISA: suitable, cell culture | mammalian: suitable

technique(s)

immunoblotting: suitable

impurities

salt, free

impurities

-

impurities

≤0.01% free fatty acid (GC)

impurities

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

General description

Tetramethylrhodamine isothiocyanate (TRITC) is stable and less sensitive to light compared to fluorescein isothiocyanate (FITC). The conjugate of albumin with TRITC is effective for following microcirculation in vivo microscopy. Albumin-TRITC aids tracking of calcium phosphate nanoparticle bound DNA in human umbilical vein endothelial cells (T-HUVEC).

Application

Albumin, Tetramethylrhodamine isothiocyanate has been used:
  • in photoinduced release assay of polyethylene beads
  • for passivating poly(methyl methacrylate) (PMMA) layer prior to immunoglobulin G coating
  • in fluorescent correlation spectroscopy

Red-fluorescing TRITC-albumin was used to track the uptake of calcium phosphate/DNA nanoparticles during cell transfection. The dye-linked albumin is incorporated into the nanoparticles. Efficiency of transfection could be followed by fluorescence microscopy.

Preparation Note

Prepared from crystallized and lyophilized albumin (A4378).

Pictograms

Exclamation markHealth hazard

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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