After packing, and before a chromatographic run, equilibrate with working buffer by washing with at least 5 bed volumes.
A25120
DEAE–Sephadex® A-25 chloride form
Synonym(s):
DEAE Sephadex®, Sephadex A 25,2-(diethylamino)ethylether, Diethylaminoethyl Sephadex®
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 10 g | Available to ship TODAYfromMILWAUKEE | $146.00 | |
| 50 g | Available to ship TODAYfromMILWAUKEE | $635.00 |
About This Item
form
powder
Quality Level
technique(s)
activity assay: suitable
matrix
cross-linked dextran
matrix active group
functional group
bead size
40-125 μm (dry)
pore size
~30,000 Da exclusion limit
operating pH
2-9
capacity
3-4 meq/g ion exchange capacity
compatibility
mode of use mixed weak and strong anion exchange chromatography
General description
The Sephadex® ion exchangers contain a cross-linked dextran matrix with functional groups. These groups are linked by stable ether linkages to the glucose units in the matrix. DEAE Sephadex can be used in ion exchange chromatography for purifying and isolating proteins.
A25120-100G′s updated product number is GE17-0170-01
A25120-500G′s updated product number is GE17-0180-02
A25120-100G′s updated product number is GE17-0170-01
A25120-500G′s updated product number is GE17-0180-02
Application
DEAE-Sephadex® A-25 has been used:
- to form DEAE-Sephadex A-25 (formate form) for glucosinolates (GLS) extraction using horseradish plants
- in the estimation of GLS content in Chinese kale sprouts
- production and purification of endozepine from astrocytes
- to identify and label an insulin-activated nitric oxide synthase inhibitor (protein) in acute myocardial infarction (AMI) patients
Features and Benefits
- Weak anion exchanger
- Suitable for batch techniques
- High binding capacities
- Non-reactive to commonly used aqueous buffers additives such as 8 M urea and 6 M guanidine hydrochloride
Legal Information
DEAE Sephadex is a registered trademark of Cytiva
Sephadex is a registered trademark of Cytiva
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This Item | |||
|---|---|---|---|
| pore size ~30,000 Da exclusion limit | pore size ~30,000 Da exclusion limit | pore size ~200,000 Da exclusion limit | pore size ~200,000 Da exclusion limit |
| matrix cross-linked dextran | matrix - | matrix - | matrix cross-linked dextran |
| matrix active group functional group | matrix active group - | matrix active group - | matrix active group - |
| operating pH 2-9 | operating pH 2-10 | operating pH 2-10 | operating pH - |
| technique(s) activity assay: suitable | technique(s) - | technique(s) - | technique(s) protein purification: suitable |
| capacity 3-4 meq/g ion exchange capacity | capacity 2.6-3.4 meq/g ion exchange capacity | capacity 2.6-3.4 meq/g ion exchange capacity | capacity 3-4 meq/g binding capacity |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Shu-Kui Wang et al.
Zhonghua nan ke xue = National journal of andrology, 8(3), 173-177 (2002-12-14)
To establish and evaluate the anti-human seminal plasma phospholipase A2 (PLA2) monoclonal antibody (McAb). After having been separated and purified from human seminal plasma by PEG precipitation, Sephacryl S-300 column chromatography, DEAE-Sephadex A-25 column chromatography and HA column chromatography, PLA2
M J Hollingsworth et al.
Molecular and cellular biology, 6(4), 1058-1064 (1986-04-01)
A requisite step in the biosynthesis of tRNA is the removal of 5' leader sequences from tRNA precursors. We have detected an RNase P activity in yeast mitochondrial extracts that can carry out this reaction on a homologous precursor tRNA.
Dong-Lin Wang et al.
Journal of agricultural and food chemistry, 50(2), 262-265 (2002-01-10)
Among 10 lipases tested, Candida rugosa lipase exhibited the best ability to catalyze the resolution of dl-menthol in organic solvent. The lipase was immobilized on different carriers, and the experiment was carried out with different acyl donors. The high yield
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A25120-500G | 04061833350409 |
| A25120-100G | 04061833350393 |
| A25120-50G | 04061833350416 |
| A25120-10G | 04061835511679 |
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How do I equilibrate Product A25120, DEAE-Sephadex® A-25 chloride form?
1 answer-
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How should I regenerate Product A25120, DEAE-Sephadex® A-25 chloride form?
1 answer-
After each separation, elute any reversibly bound material either with a high ionic strength solution (e.g. 1 M NaCl in buffer) or by increasing pH. Regenerate the media by washing with at least 5 bed volumes of buffer, or until the column effluent shows stable conductivity and pH values.
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What is the chemical stability of Product A25120, DEAE-Sephadex® A-25 chloride form?
1 answer-
According to the manufacturer, this is compatible with all commonly used aqueous buffers, 1 M NaOH, 8 M urea, 6 M guanidine hydrochloride and 70% ethanol.
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How should I store Product A25120, DEAE-Sephadex® A-25 chloride?
1 answer-
Unused media can be stored in the container at +4 to +30 °C. Ensure that the screw-top is fully tightened. Packed columns should be equilibrated in working buffer containing 20% ethanol to prevent microbial growth.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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