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A2543

Sigma-Aldrich

Anti-α-Actinin antibody produced in rabbit

whole antiserum

Synonym(s):
Anti-CMD1AA, Anti-CMH23, Anti-MPD6, Anti-MYOCOZ
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

contains

15 mM sodium azide

species reactivity

chicken

technique(s)

indirect immunofluorescence: 1:500 using cultured chicken fibroblasts
western blot: suitable using cultured cells and chicken gizzard extract

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

General description

α-Actinin is a 100kDa actin-binding protein that is found in muscle as well as non-muscle cells. Rabbit anti-α-actinin antibody stains α-actinin in cell cultures using indirect immunofluorescence. The antibody reacts best with cultured chicken fibroblasts, however, adequate labeling may also be obtained with cells of other species. In an immunoblot assay, the antiserum specifically stains the α-actinin band.
α-Actinin is a member of the spectrin superfamily. It consists of an antiparallel homodimer, with an N-terminal actin-binding domain (ABD), a central domain of four spectrin-like repeats (SRs), and a C-terminal calmodulin-like domain (CAMD) with two pairs of EF-hand motifs (EFs). α-Actinin exists in four isoforms: α-actinin 1, α-actinin 2, α-actinin 3 and α-actinin 4. In smooth muscles, α-actinin is present in dense bodies and plaques whereas, in normal skeletal muscles, it is associated with Z-discs that define muscle sarcomere.

Specificity

Anti-α-Actinin specifically stains α-actinin in cell cultures using indirect immunfluorescent labeling techniques. The antibody reacts best with cultured chicken fibroblasts, however adequate labeling may also be obtained with cells of other species.

Immunogen

α-actinin from chicken gizzard

Application

Anti-Actinin antibody was used to detect actinin in human mesangial cells. Cells were first grown on glass cover slips, fixed in cold methanol and permeabilized with 3.5% paraformaldehyde/0.1% Tween-20 for 5 min at room temperature.
Rabbit anti-α-actinin antibody has been used for:
  • co-immunoprecipitation studies
  • immunoblotting studies
  • immunofluorescence

Biochem/physiol Actions

α-Actinin is an actin-crosslinking Z-disk protein. It plays a vital role in linking the cytoskeleton to different transmembrane proteins in a variety of junctions. α-Actinin modulates receptor activities and serves as a cytoskeleton scaffold for diverse signaling pathways. It is implicated in muscle contraction.

Physical form

Supplied as a liquid with 0.1% sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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SDS

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