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A2856

Sigma-Aldrich

Anti-ATG7 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Synonym(s):
ATG7 Antibody - Anti-ATG7 antibody produced in rabbit, Atg7 Antibody, Anti-APG7, Anti-APG7-like, Anti-APG7L, Anti-Apoptosis-specific-protein, Anti-Autophagy protein 7-like, Anti-Ubiquitin activating enzyme E1-like protein
MDL number:
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~75 kDa

species reactivity

rat, human, mouse

packaging

antibody small pack of 25 μL

technique(s)

immunoprecipitation (IP): 1-2 μL using human U87 cell lysate
western blot: 0.5-1 μg/mL using whole extracts of mouse 3T3 cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ATG7(10533)
mouse ... Atg7(74244)
rat ... Atg7(312647)

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This Item
A3231HPA007639SAB5700649
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous glycerol solution

form

buffered aqueous solution

mol wt

antigen ~75 kDa

mol wt

antigen ~36 kDa

mol wt

-

mol wt

68, 75, 77

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General description

Autophagy is a catabolic process by which cell delivers cytoplasmic constituent into lysosome for protein degradation. 16 ATG genes are essential for autophagosome preparation out of which Atg7 is the one required to activate ubiquitin-like proteins Atg12 and Atg8. Atg7 is also crucial for amino acid supply in neonates, as well as for bulk degradation of proteins and organelles in mice during starvation. Anti-ATG7 antibody can be used in immunohistochemistry and immunoprecipitation. Rabbit anti-ATG7 antibody reacts specifically with Atg7 of human, mouse and rat.

Immunogen

synthetic peptide corresponding to amino acids 38-50 of human ATG7, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in mouse and rat.

Application

Anti-ATG7 antibody can be used in western blotting and immunoblotting.
Anti-ATG7 antibody produced in rabbit has been used:
  • for quantitative real-time polymerase chain reaction of autophagy-related genes
  • in immunofluorescence and immunoblotting
  • in in vivo acetylation/deacetylation of autophagy proteins

Rabbit anti-ATG7 antibody has been used for immunoblotting assays. The antibody is also suitable for use in immunoprecipitation applications at 1-2μL using human U87 cell lysates.
Rabbit anti-ATG7 antibody was used for immunohistochemistry on sections of mouse midbrain fixed in 4% paraformaldehyde at 1:2000.

Biochem/physiol Actions

Autophagy related 7 (ATG7) regulates caspase mediated autophagy and apoptosis. This has a significant effect on tumor progression and ATG7 acts as a tumor suppressor. ATG7 is known to be associated with many cancer types, hence it is considered as a target for cancer therapy. Loss of ATG7 gene contributes to liver carcinogenesis. The ATG7 gene encodes E1-like enzyme essential for lipidation and conjugation systems : ubiquitin-like microtubule-associated protein 1A/1B-light chain 3 (LC3) and autophagy related 12 (ATG12) respectively.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Midbody ring disposal by autophagy is a post-abscission event of cytokinesis
Pohl C and Jentsch S
Nature Cell Biology, 11(1), 65-65 (2009)
Man J Livingston et al.
Autophagy, 12(6), 976-998 (2016-04-29)
Renal fibrosis is the final, common pathway of end-stage renal disease. Whether and how autophagy contributes to renal fibrosis remains unclear. Here we first detected persistent autophagy in kidney proximal tubules in the renal fibrosis model of unilateral ureteral obstruction
Jessie Yanxiang Guo et al.
Genes & development, 27(13), 1447-1461 (2013-07-05)
Macroautophagy (autophagy hereafter) degrades and recycles proteins and organelles to support metabolism and survival in starvation. Oncogenic Ras up-regulates autophagy, and Ras-transformed cell lines require autophagy for mitochondrial function, stress survival, and engrafted tumor growth. Here, the essential autophagy gene
PSMD10/gankyrin induces autophagy to promote tumor progression through cytoplasmic interaction with ATG7 and nuclear transactivation of ATG7 expression
Luo T, et al.
Autophagy, 12(8), 1355-1371 (2016)
The emerging links between sirtuins and autophagy
Sirtuins, 259-271 (2013)

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