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MilliporeSigma

A2901

Sigma-Aldrich

Avidin–FITC from egg white

lyophilized powder

Synonym(s):

Avidin–Fluorescein isothiocyanate from egg white

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About This Item

MDL number:
MFCD00130585
UNSPSC Code:
12352203
NACRES:
NA.46

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conjugate

FITC conjugate

Quality Level

200

form

lyophilized powder

extent of labeling

2-4 mol fluorescein isothiocyanate per mol avidin

technique(s)

immunohistochemistry: suitable

storage temp.

−20°C

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This Item
A9275A2050A3151
Avidin–FITC from egg white lyophilized powder

Sigma-Aldrich

A2901

Avidin–FITC from egg white

Avidin from egg white BioUltra, lyophilized powder, ≥10 units/mg protein (E1%/280), ≥98% (SDS-PAGE)

Sigma-Aldrich

A9275

Avidin from egg white

Avidin–FITC from egg white buffered aqueous solution

Sigma-Aldrich

A2050

Avidin–FITC from egg white

Avidin–Peroxidase lyophilized powder

Sigma-Aldrich

A3151

Avidin–Peroxidase

technique(s)

immunohistochemistry: suitable

technique(s)

-

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-40 μg/mL, indirect immunofluorescence: 1:200

technique(s)

direct ELISA: 1:50,000

form

lyophilized powder

form

lyophilized powder

form

buffered aqueous solution

form

lyophilized powder

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

conjugate

FITC conjugate

conjugate

-

conjugate

FITC conjugate

conjugate

peroxidase conjugate

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

extent of labeling

2-4 mol fluorescein isothiocyanate per mol avidin

extent of labeling

-

extent of labeling

3-5.5 mol fluorochrome per mol protein

extent of labeling

0.7-2.0 mol peroxidase per mol avidin

General description

Avidin is homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues[1]. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

Application

Avidin-FITC from egg white has been used for immunohistochemistry applications[2].
To determine the amount of labeled polyclonal IgG present in spores in an IgG prep. A known concentration of FITC avidin from egg white and biotin-IgG avidin was used in excess at 300nm.[3]

Packaging

Package size based on protein content

Physical form

Contains sodium citrate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000464721
0000464725
0000464721
0000464725
SLCQ3738

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Sergey Mursalimov et al.
Protoplasma, 254(3), 1379-1384 (2016-09-30)
Cytomixis is a poorly studied process of nuclear migration between plant cells, discovered in microsporogenesis of several hundreds of plant species. The chromosomes that migrate between tobacco microsporocytes have been for the first time identified using fluorescence in situ hybridization
J C Sørensen et al.
Experimental brain research, 112(2), 203-212 (1996-11-01)
Thalamic neuronal degeneration after neocortical lesions involve both anterograde and retrograde components. This study deals with the thalamic microglial response after neocortical aspiration lesions, using fluorogold fluorescent prelabeling, to identify retrogradely degenerating thalamocortical neurons, combined with histochemical or immunohistochemical staining
Lizanne G Nilewski et al.
ACS chemical neuroscience, 9(1), 107-117 (2017-07-29)
Selective targeting of drug loaded nanovectors to specific epitopes highly expressed on the surface of cancer cells is a goal for nanotechnologists. We have modified our previously described PEGylated-hydrophilic carbon clusters (PEG-HCCs) so that the epidermal growth factor receptor (EGFR)
S M Hsu et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 29(4), 577-580 (1981-04-01)
The use of avidin-biotin interaction in immunoenzymatic techniques provides a simple and sensitive method to localize antigens in formalin-fixed tissues. Among the several staining procedures available, the ABC method, which involves an application of biotin-labeled secondary antibody followed by the

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