A2917

Acrylamide/Bis-acrylamide

BioReagent, 19:1 (ratio), Molecular Biology

• Synonym(s): Acrylamide/Bisacrylamide, 19:1 (5% crosslinker), Polyacrylamide solution
• MDL number: MFCD00080848
• UNSPSC Code: 41105319
• PubChem Substance ID: 329770804
• NACRES: NA.25

Properties

• grade: Molecular Biology
• product line: BioReagent
• form: powder
• feed ratio: 19:1 (ratio)
• foreign activity: DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected
• SMILES string: NC(=O)C=C.C=CC(=O)NCNC(=O)C=C
• InChI: 1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)
• InChI key: OCQZXMCGTAWGEQ-UHFFFAOYSA-N

Description

General description

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent.[1] On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.[2]

Application

Acrylamide/Bis-acrylamide has been used:

• in the preparation of urea polyacrylamide gel during a modified cross-linking affinity purification protocol (cCLAP)[3]
• in microsatellite genotyping and nonfluorescent elastin (ELN) (GT)n polymorphism genotyping[4]
• in polyacrylamide gel for separation of proteins in SDS-PAGE for western blotting[5]

Ready to reconstitute dry powder blends are accurately pre-blended to produce a 40% (w/v) stock solution for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Features and Benefits

Eliminates the need to weigh toxic acrylamide and bis-acrylamide.

Preparation Note

Reconstitute by slowly adding the indicated amount of distilled water to the container and dissolve contents.

To make up 100 ml of A2917, slowly add 63 ml distilled or deionized water to the container and dissolve contents. Do not divide the solid. This makes 100 ml of a 40% stock solution.
For the 1 Liter size, use 630 mL of water.

Safety Information

• pictograms: GHS06,GHS08
• signalword: Danger
• hcodes: H301,H312 + H332,H315,H317,H319,H340,H350,H361fd,H372
• pcodes: P202 - P280 - P301 + P310 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral
• target_organs: Peripheral nervous system
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges