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A2981

Sigma-Aldrich

Anti-ATG4B antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Synonym(s):
Anti-APG4B, Anti-AUT-like 1 cysteine endopeptidase, Anti-AUTL1, Anti-Autophagin-1, Anti-Autophagy-related cysteine endopeptidase 1, Anti-Autophagy-related protein 4 homolog B, Anti-Cysteine protease ATG4B, Anti-KIAA0943, Anti-hAPG4B, ATG4
MDL number:
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~44 kDa

species reactivity

mouse, rat, human

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 using biotin/ExtaAvidin®-Peroxidase staining of heat-retrieved human cerebellum sections
western blot: 1:125-1:500 using whole extract of 3T3 cells
western blot: 1:500-1:1,000 using whole extract of rat brain

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ATG4B(23192)
mouse ... Atg4b(66615)
rat ... Atg4b(316640)

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This Item
SAB5700787HPA036374HPA069803
conjugate

unconjugated

conjugate

-

conjugate

unconjugated

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

mol wt

antigen ~44 kDa

mol wt

31, 37, 42, 44, 52

mol wt

-

mol wt

-

General description

ATG4B (autophagy-related 4B cysteine peptidase), a mammalian orthologue of yeast Atg4, is a cysteine protease. It is widely expressed in human and rat tissues, being most abundant in the brain. This gene is mapped to human chromosome 2q37.
ATG4B is a member of the C-54 family of cysteine proteases that is involved in cell degradation through autophagy.

Immunogen

synthetic peptide corresponding to amino acids 6-22 of human ATG4B, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in mouse.

Application

Anti-ATG4B antibody produced in rabbit has been used in
  • western blotting
  • immunohistochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

Biochem/physiol Actions

ATG4B (autophagy-related 4B cysteine peptidase) participates in the modulation of autophagy in mammalian cells. It is also involved in light chain 3 (LC3) lipidation. ATG4B is responsible for the processing of the mammalian Atg8 homologues: Golgi-associated ATPase enhancer of 16 kDa (GATE16), gamma-aminobutyric-acid-type-A-receptor-associated protein (GABARAP), and LC3. It removes the carboxyl-terminal region of proLC3 immediately after its synthesis, generating the soluble LC3-I and exposing a carboxyl-terminal Gly.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Identification of breast cancer cell subtypes sensitive to ATG4B inhibition
Bortnik S, et al.
Testing, 7(41), 66970-66970 (2016)
AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells
Ni Z, et al.
Autophagy, 14(4), 685-701 (2018)
O-GlcNAcylation of ATG4B positively regulates autophagy by increasing its hydroxylase activity
Jo YK, et al.
Implant Dentistry, 7(35), 57186-57186 (2016)
Let-7i-Induced Atg4B Suppression Is Essential for Autophagy of Placental Trophoblast in Preeclampsia
Xu Y, et al.
Journal of Cellular Physiology, 232(9), 2581-2589 (2017)
Essential role for the ATG4B protease and autophagy in bleomycin-induced pulmonary fibrosis
Cabrera S, et al.
Autophagy, 11(4), 670-684 (2015)

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