A3156

Sigma-Aldrich

Bovine Serum Albumin

lyophilized powder, γ-irradiated, Globulin Free, BioXtra, suitable for cell culture

Synonym(s):
Albumin bovine serum, Bovine albumin, BSA
CAS Number:
EC Number:
MDL number:
NACRES:
NA.75

Quality Level

biological source

bovine

sterility

γ-irradiated

product line

BioXtra

assay

≥99% (agarose gel electrophoresis)

form

lyophilized powder

mol wt

~66 kDa

purified by

cold ethanol and heatshock fractionation

application(s)

cell culture | mammalian: suitable

pH

4.8-7.5

solubility

water: soluble (5g/50ml)

shipped in

ambient

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

General description

BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.
Bovine serum albumin (BSA) is a 66 kDa protein consisting of three domains with two subdomains under each. It is a α-helical, globular and non-glycosylated protein. Albumin is the most abundant plasma protein in humans.

Application

Bovine serum albumin has been used:
  • to prevent sticking of the chromosome to the glass micropipette for elasticity measurements using aspiration
  • to block lung homogenates in the plate for mucin protein enzyme-linked immunosorbent assay
  • in alginate solution as a protein model for drug release kinetics investigation

Biochem/physiol Actions

Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Bovine Serum Albumin (BSA) is a transporter for drugs, hormones and fatty acids. Albumin turnover is seen in infants with iron deficiency anemia. BSA acts as a vital constituent in the cell culture media and favors human embryonic stem cells (hESC) differentiation.

Preparation Note

Purified by a combination of heat shock and ethanol fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Elasticity and Structure of Eukaryote Chromosomes Studied by Micromanipulation and Micropipette Aspiration
Houchmandzadeh B, et al.
The Journal of cell biology, 139(1), 1-12 (1997)
Acrolein-Activated Matrix Metalloproteinase 9 Contributes to Persistent Mucin Production
Deshmukh HS, et al.
American Journal of Respiratory Cell and Molecular Biology (2012)
Combination of polymer technology and carbon nanotube array for the development of an effective drug delivery system at cellular level
Riggio C, et al.
Nanoscale Research Letters, 4(7), 668-668 (2009)
Albumin and mammalian cell culture: implications for biotechnology applications
Francis GL
Cytotechnology, 62(1), 1-16 (2010)
Bovine serum albumin interactions with metal complexes
Topalua T, et al.
Clujul Medical (1957), 87(4), 215-215 (2014)
Articles
This page segues to comprehensive insights on how serum albumin and other important cell culture components affect the performance of serum-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of albumin. These insights may also be applied to ex vivo and tissue engineering applications.
Read More
Protocols
cAMP measurements are obtained using an ELISA assay (Harlow and Lane 1988). Commercial radio-immunoassays, or ELISA kits, to assay cAMP can be purchased from various manufacturers.
Read More

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