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MilliporeSigma

A3176

α-Amylase from porcine pancreas

greener alternative

Type VI-B, ≥5 units/mg solid

Synonym(s):

alpha-Amylase, β-N-acetylglucosaminidase porcine placenta, PPA, al1,4 glucan-4-glucanohydrolase,, porcine pancreas α-amylase

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500000 UNITS

$65.80

1000000 UNITS

$95.50

2500000 UNITS

$194.00

5000000 UNITS

$312.00

10000000 UNITS

$482.00

$65.80

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About This Item

UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-565-6
NACRES:
NA.54
MDL number:
MFCD00081319
EC Number:
3.2.1.1 (BRENDA, IUBMB)
Specific activity:
≥5 units/mg solid
Biological source:
Porcine pancreas
Concentration:
~90% (lactose)

Key Documents

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biological source

Porcine pancreas

type

Type VI-B

form

powder

specific activity

≥5 units/mg solid

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

concentration

~90% (lactose)

technique(s)

activity assay: suitable

suitability

suitable for hydrolysis, synthesis of oligosaccharides and polysaccharides, and sugar modification

application(s)

life science and biopharma

greener alternative category

Enabling,

storage temp.

2-8°C

Quality Level

200

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1 of 4

This Item
A6255A4268A9857
α-Amylase from porcine pancreas Type VI-B, ≥5 units/mg solid

Sigma-Aldrich

A3176

α-Amylase from porcine pancreas

α-Amylase from porcine pancreas PMSF Treated, Type I-A, saline suspension, ≥1000 units/mg protein (E1%/280)

Sigma-Aldrich

A6255

α-Amylase from porcine pancreas

α-Amylase from porcine pancreas Type I-A, PMSF treated, saline suspension, 700-1400 units/mg protein (E1%/280)

Sigma-Aldrich

A4268

α-Amylase from porcine pancreas

α-Amylase from Aspergillus oryzae ≥150 units/mg protein (biuret)

Sigma-Aldrich

A9857

α-Amylase from Aspergillus oryzae

biological source

Porcine pancreas

biological source

Porcine pancreas

biological source

Porcine pancreas

biological source

-

technique(s)

activity assay: suitable

technique(s)

-

technique(s)

activity assay: suitable

technique(s)

-

specific activity

≥5 units/mg solid

specific activity

≥1000 units/mg protein (E1%/280)

specific activity

700-1400 units/mg protein (E1%/280)

specific activity

≥150 units/mg protein (biuret)

suitability

suitable for hydrolysis, synthesis of oligosaccharides and polysaccharides, and sugar modification

suitability

-

suitability

suitable for hydrolysis, synthesis of oligosaccharides and polysaccharides, and sugar modification

suitability

-

concentration

~90% (lactose)

concentration

-

concentration

-

concentration

-

form

powder

form

saline suspension

form

saline suspension

form

powder

Analysis Note

Contains lactose

Application

α-Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. α-Amylase, from Sigma, has been used in various plant studies, such as metabolism studies in Arabidopsis [1]. α-Amylase, from porcine pancreas, has been used to remove starch during the study of starch metabolism and ripening in tomatoes [2].

Biochem/physiol Actions

α-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. α -Amylase, from porcine pancreas, is a glycoprotein that consists of a single polypeptide chain of approximately 475 residues containing 2 SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability. The pH optimum is at 7.

General description

Molecular mass: 51-54 kDa.
α-Amylase isolated from porcine pancreas is a glycoprotein. It is a single polypeptide chain of ~475 residues containing two SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability. Chloride ions are necessary for activity and stability. The pH range for activity is 5.5 to 8.0, with the pH optimum at 7.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Other Notes

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Packaging

Package size based on α-amylase activity

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000526123
0000526124
0000526124
0000526123
0000508022

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Haoxin Li
Food science & nutrition, 8(7), 3252-3261 (2020-07-30)
Butternut squash is greatly consumed in United Kingdom and as by-products of its processing are produced large amounts of skin and seeds. However, little research has been reported on the antioxidant properties and bioactive peptides from butternut squash seeds and
Amir Amini Khoozani et al.
Foods (Basel, Switzerland), 9(2) (2020-02-09)
The use of Whole Green Banana Flour (WGBF) in bread production may be a strategy to improve the nutritional profile of bread, but the extent of improvement may depend on the processing conditions of the flour. Therefore, WGBF was produced
Massimiliano Carciofi et al.
BMC plant biology, 12, 223-223 (2012-11-23)
Starch is stored in higher plants as granules composed of semi-crystalline amylopectin and amorphous amylose. Starch granules provide energy for the plant during dark periods and for germination of seeds and tubers. Dietary starch is also a highly glycemic carbohydrate
Xin Huang et al.
Foods (Basel, Switzerland), 9(7) (2020-07-28)
The ingestion of gluten-containing foods can cause wheat-related disorders in up to 15% of wheat consuming populations. Besides the role of gluten, α-amylase/trypsin inhibitors (ATI) have recently been identified as inducers of an innate immune response via toll-like receptor 4
Seyadeh Narges Mazloomi et al.
Foods (Basel, Switzerland), 9(9) (2020-09-06)
In this study, orange seed proteins were hydrolyzed by Alcalase enzyme at different enzyme concentrations 1-3% (v/w) and hydrolysis times (2-5 h), to obtain bioactive peptides showing antioxidant, Angiotensin-converting enzyme (ACE) -inhibitory, and hypoglycemic activities. The highest biological activities (p
View All Related Papers

Protocols

Enzymatic Assay of α-Amylase (EC 3.2.1.1)

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

Questions

1–5 of 5 Questions  

  1. What is the recommended buffer and temperature that results in the optimal enzyme activity of this enzyme?

    1 answer

    1. For testing purposes, this product is prepared at 0.75?1.5 units/mL in water. The test is run at 20 °C. Please see the link below to review the assay procedure, which includes additional buffer formulations and standard preparation instructions:
      https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-a-amylase

      Please see the following link to review addtional information available in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/348/718/a3176pisrev0924-ms.pdf

      Helpful?

  2. The product is insoluble in 0.05 M Phosphate buffer and water. What is the solubility of the product

    1 answer

    1. This product is not specifically tested for solubility. However, as mentioned on the Product Datasheet, the material is expected to be soluble at 0.5 mg/mL in 20mM phosphate-buffered saline containing 6mM Sodium Chloride, pH 6.9. This information is according to the following reference:
      Irondi, F.A. et al., Food Sci. Nutr., 5(2), 233-242 (2017).

      For analytical purposes, this enzyme is prepared at 1 unit/mL in sold deionized water in the determination of enzymatic activity.

      Please see the link below to review the product datasheet for additional information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/966/306/a3176pis.pdf

      Helpful?

  3. What type of amylase will work effectively with Benedicts reagents for a digestion experiment?

    1 answer

    1. The alpha amylase products have not been tested with Benedict's Reagent to confirm the release of various reducing sugars resulting from starch digestion.

      Helpful?

  4. Could you please provide the approximate weight in grams of the product A3176-500KU?

    1 answer

    1. The product with code A3176-500KU has an approximate weight of 5 grams. This is calculated as follows: 50000 x 1mg / 10 units = 5000mg, and then 5000mg x 1g / 1000mg = 5g.

      Helpful?

  5. What is the optimal temperature for this enzyme?

    1 answer

    1. The enzymatic activity testing of this product is performed at room temperature. This enzyme has a broad temperature range with various publications citing activity from 10 - 80°C, at neutral pH. Please see the links below to review the product datasheet, the enzyme assay protocol, and a sample Certificate of Analysis for additional information:

      Product datasheet
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/966/306/a3176pis.pdf

      Product enzyme assay
      https://www.sigmaaldrich.com/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-a-amylase

      Sample Certificate of Analysis
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/A3176-BULKSLCQ1770.pdf

      Helpful?

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