A3188

Sigma-Aldrich

Anti-Human IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

MDL number:

biological source

goat

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

species reactivity

human

should not react with

mouse

application(s)

direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000

conjugate

alkaline phosphatase conjugate

shipped in

wet ice

storage temp.

2-8°C

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General description

Human IgG antibodies are primarily responsible for the facilitation of humoral immune responses such as complement fixation, placental transport, and phagocytosis. Anti-human IgG (γ-chain specific) −alkaline phosphatase antibody can be used in immunohistochemistry and immunoelectrophoresis. It can also be used in western blotting. Goat anti-human IgG−alkaline phosphatase antibody reacts specifically with human IgG when tested against human IgA, IgG, IgM, Bence Jones κ, and λ myeloma proteins. The product is not reactive with mouse IgG or mouse serum proteins.

Immunogen

Purified human IgG.

Application

Proteins harvested from E. coli cultures were run on SDS-page gel for western blot analysis using alkaline phosphatase conjugated goat anti-human IgG (chain specific) at a concentration of 1:10000 for the secondary. IgG was detected in human serum by Elisa using alkaline phosphatase conjugated goat anti-human IgG (gamma-chain specific) diluted 1:5000 in 0.25% BSA/PBS. Assay was developed using p-nitrophenyl phosphate substrate (1mg/ml Sigma).
Anti-human IgG (γ-chain specific) −alkaline phosphatase antibody can be used in immunocytochemical staining techniques. It can also be used for dot blot.

Other Notes

Antibody adsorbed with mouse serum proteins.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide as preservative

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
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