A3525

Sigma-Aldrich

Anti-Human IgE (ε-chain specific)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

MDL number:
NACRES:
NA.46
Pricing and availability is not currently available.

Quality Level

biological source

goat

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

species reactivity

human

application(s)

direct ELISA: 1:7,000-1:21,000

conjugate

alkaline phosphatase conjugate

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

Affinity isolated antibody is obtained from anti-human IgE antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the ε-chain of human IgE. Anti-Human IgE is conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde. IgE is present in low levels compared to other immunoglobulin isotypes. It exists in two forms, one being a secreted by plasma cell and the other form is associated with the B cell receptor.
IgEs are glycoprotein antibodies that regulate immunological responses to allergies and infections. These immunoglobulins have been implicated in mediating Type I hypersensitivity reactions. Elevated IgE levels have been associated with hyper IgE syndrome and allergic asthma . Anti-Human IgE (epsilon-chain specific)-Alkaline Phosphatase antibody is specific for human IgE when tested against human IgA, IgG, IgM, Bence Jones κ and λ myeloma proteins.

Immunogen

Purified human IgE

Application

Anti-Human IgE (ε-chain specific)−Alkaline Phosphatase antibody produced in goat has been used in dot blot.
Anti-Human IgE (epsilon-chain specific)-Alkaline Phosphatase antibody is suitable for use in immunoblotting and ELISA (at 1:500 dilutions using 100μ/well).
IgE secreted from transfected murin myeloma cells was detected by ELISA using the alkaline phosphatase-conjugated goat anti-human IgE (epsilon specific) antibody.

Biochem/physiol Actions

IgE bound to B cell receptor mediates antigen uptake and regulates differentiation and proliferation of B cells.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 15 mM sodium azide and 50% glycerol

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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C D Lindstrøm et al.
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