Skip to Content
MilliporeSigma

A3547

Achromopeptidase from bacteria

lyophilized powder, ≥1,000 units/mg solid

Synonym(s):

ACP, Lysyl endopeptidase

Slide 1 of 2
Sign In to View Organizational & Contract Pricing

Select a Size

100000 UNITS

$197.00

500000 UNITS

$804.00

1000000 UNITS

$1,350.00

$197.00

Check Cart for Availability
Request a Bulk Order

About This Item

UNSPSC Code:
12352204
NACRES:
NA.54

Key Documents

View All Documentation

Skip To

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

bacterial (LYSOBACTER SP. BACTERIA M497-1)

form

lyophilized powder

specific activity

≥1,000 units/mg solid

mol wt

27 kDa

technique(s)

cell based assay: suitable

suitability

suitable for cell lysis

application(s)

diagnostic assay manufacturing

shipped in

dry ice

storage temp.

−20°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
A3422SRE0041SRE0053
Achromopeptidase from bacteria lyophilized powder, ≥1,000 units/mg solid

Sigma-Aldrich

A3547

Achromopeptidase from bacteria

Achromopeptidase from bacteria partially purified powder, ≥20,000 units/mg solid

Sigma-Aldrich

A3422

Achromopeptidase from bacteria

Catalase from bovine liver Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, 2,000-5,000 units/mg protein

Sigma-Aldrich

SRE0041

Catalase from bovine liver

Lysostaphin from Staphylococcus staphylolyticus Suitable for manufacturing of diagnostic kits and reagents., lyophilized powder

Sigma-Aldrich

SRE0053

Lysostaphin from Staphylococcus staphylolyticus

technique(s)

cell based assay: suitable

technique(s)

-

technique(s)

-

technique(s)

-

biological source

bacterial (LYSOBACTER SP. BACTERIA M497-1)

biological source

-

biological source

-

biological source

bacterial (Staphylococcus sp.)

specific activity

≥1,000 units/mg solid

specific activity

≥20,000 units/mg solid

specific activity

2,000-5,000 units/mg protein

specific activity

≥500 units/mg protein

suitability

suitable for cell lysis

suitability

-

suitability

-

suitability

suitable for microbiological tests

form

lyophilized powder

form

partially purified powder

form

lyophilized powder

form

lyophilized powder

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

Application

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections[1].

Biochem/physiol Actions

Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD600 = 0.6 over 2 hours at 37 °C.
Achromopeptidase is a lysyl endopeptidase with a MW of ~27 kDa. It is useful for lysis of Gram-positive bacteria that are resistant to lysozyme.
pH Optimum for activity: pH 8.5 - 9
Approximately 500-1,500 un/ml achromopetidase can be used to lyse cells at a density of OD600=0.6 over 2 hours at 37 °C.

Physical form

crude powder

Other Notes

One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000506234
0000506234
0000491295
0000491294
0000491294

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

J R Buser et al.
Analytical methods : advancing methods and applications, 8(14), 2880-2886 (2016-04-14)
Sample preparation, including bacterial lysis, remains a hurdle in the realization of complete point-of-care tests for many pathogens. Here, we developed a sample preparation methodology for enzymatic lysis and sample heating for low-resource, point-of-care applications. We show an instrument-free chemical
Georgios Chondrogiannis et al.
Scientific reports, 11(1), 6140-6140 (2021-03-19)
Enzymes are the cornerstone of modern biotechnology. Achromopeptidase (ACP) is a well-known enzyme that hydrolyzes a number of proteins, notably proteins on the surface of Gram-positive bacteria. It is therefore used for sample preparation in nucleic acid tests. However, ACP
Disposable platform for bacterial lysis and nucleic acid amplification based on a single USB-powered printed circuit board.
Shah, et al.
PLoS ONE, 18, e0284424-e0284424 (2023)
Adebayo Shittu et al.
Journal of medical microbiology, 53(Pt 1), 51-55 (2003-12-10)
The isolation, molecular identification and genotyping of multiresistant Staphylococcus sciuri and Staphylococcus haemolyticus from skin and soft-tissue infections are reported. Accurate and full identification of three coagulase-negative staphylococcal isolates was achieved using PCR, while the API STAPH method failed to
Chelsey A Smith et al.
Lab on a chip, 23(3), 451-465 (2022-12-24)
Cervical cancer is a leading cause of cancer death for women in low-resource settings. The World Health Organization recommends that cervical cancer screening programs incorporate HPV DNA testing, but available tests are expensive, require laboratory infrastructure, and cannot be performed
View All Related Papers

Protocols

Enzymatic Assay of Achromopeptidase

To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.

Related Content

Cell Lysis Buffers & Protein Extraction Reagents

Find cell lysis and protein extraction reagents like cell lysis buffers, protein extraction kits, extraction enzymes, and other lysis enhancers for isolating and purifying proteins.

Buffer de lisis celular y reactivos de extracción de proteínas

Encuentre reactivos de lisis celular y extracción de proteínas como tampones de lisis celular, kits de extracción de proteínas, enzimas de extracción y otros potenciadores de lisis para aislar y purificar proteínas.

Achromopeptidase from Achromobacter lyticus (A3547)

QC Methods

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service