All Photos(4)

A3553

Sigma-Aldrich

Acrylamide

suitable for electrophoresis, ≥99% (HPLC), powder

Synonym(s):
2-Propenamide, Acrylic acid amide
Linear Formula:
CH2=CHCONH2
CAS Number:
Molecular Weight:
71.08
Beilstein:
605349
EC Number:
MDL number:
eCl@ss:
39031205
PubChem Substance ID:
NACRES:
NB.22

Quality Level

vapor density

2.45 (vs air)

vapor pressure

0.03 mmHg ( 40 °C)

assay

≥99% (HPLC)

form

powder

application(s)

electrophoresis: suitable

impurities

≤0.005% Acrylic acid
≤0.005% Insoluble in water

conductivity

≤10 μmho (35% solution)

bp

125 °C/25 mmHg (lit.)

mp

82-86 °C (lit.)

solubility

water: soluble 2.5 g/10 mL, clear, colorless

cation traces

Fe: ≤1 ppm
Mg: ≤3 ppm
heavy metals (as Pb): ≤5 ppm

SMILES string

NC(=O)C=C

InChI

1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)

InChI key

HRPVXLWXLXDGHG-UHFFFAOYSA-N

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General description

Acrylamide functions as a quencher of tryptophan fluorescence. Acrylamide along with bis-acrylamide crosslinks to form polyacrylamide gels, which is used to separate protein and other biomolecules. Bis-acrylamide is essential for crosslinking and acrylamide is required for the polymerization of monomers. Acrylamide alkylates SH groups of cysteine and ε-NH2 lysine residues of proteins during electrophoresis.

Application

Acrylamide has been used in the preparation of polyacrylamide gels.

Packaging

100, 500 g in glass bottle
1 kg in glass bottle

Compatibility

This substance has been tested against several types of hand protection for CE compliance. See Related Products to find the recommended gloves for handling this product.

Pictograms

Skull and crossbonesHealth hazard

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

Target Organs

Peripheral nervous system

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

280.4 °F - closed cup

Flash Point(C)

138 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

I-Chien Liao et al.
Advanced functional materials, 23(47), 5833-5839 (2014-03-01)
The development of synthetic biomaterials that possess mechanical properties that mimic those of native tissues remains an important challenge to the field of materials. In particular, articular cartilage is a complex nonlinear, viscoelastic, and anisotropic material that exhibits a very...
The Protein Protocols Handbook (2007)
Relationships among cell morphology, intrinsic cell stiffness and cell-substrate interactions
Chiang MYM, et al.
Biomaterials, 34(38), 9754-9762 (2013)
Chemistry, biochemistry, and safety of acrylamide. A review
Friedman M
Journal of Agricultural and Food Chemistry, 51(16), 4504-4526 (2003)
Janneke G F Hogervorst et al.
Critical reviews in toxicology, 40(6), 485-512 (2010-02-23)
Since 2002, it is known that the probable human carcinogen acrylamide is present in commonly consumed carbohydrate-rich foods, such as French fries and potato chips. In this review, the authors discuss the body of evidence on acrylamide carcinogenicity from both...

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