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A3553

Sigma-Aldrich

Acrylamide

suitable for electrophoresis, ≥99% (HPLC), powder

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Synonym(s):
2-Propenamide, Polyacrylamide, acrylic amide, akrylamid, Acrylic acid amide
Linear Formula:
CH2=CHCONH2
CAS Number:
Molecular Weight:
71.08
Beilstein:
605349
EC Number:
MDL number:
eCl@ss:
39031205
PubChem Substance ID:
NACRES:
NB.22

vapor density

2.45 (vs air)

Quality Level

vapor pressure

0.03 mmHg ( 40 °C)

Assay

≥99% (HPLC)

form

powder

technique(s)

electrophoresis: suitable

impurities

≤0.005% Acrylic acid
≤0.005% Insoluble in water

conductivity

≤10 μmho (35% solution)

bp

125 °C/25 mmHg (lit.)

mp

82-86 °C (lit.)

solubility

water: soluble 2.5 g/10 mL, clear, colorless

cation traces

Fe: ≤1 ppm
Mg: ≤3 ppm
heavy metals (as Pb): ≤5 ppm

SMILES string

NC(=O)C=C

InChI

1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)

InChI key

HRPVXLWXLXDGHG-UHFFFAOYSA-N

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This Item
A888723701A9099
Acrylamide suitable for electrophoresis, ≥99% (HPLC), powder

Sigma-Aldrich

A3553

Acrylamide

Acrylamide suitable for electrophoresis, ≥99%

Sigma-Aldrich

A8887

Acrylamide

Acrylamide analytical standard

Supelco

23701

Acrylamide

Acrylamide for molecular biology, ≥99% (HPLC)

Sigma-Aldrich

A9099

Acrylamide

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

-

impurities

≤0.005% Acrylic acid, ≤0.005% Insoluble in water

impurities

-

impurities

-

impurities

≤0.005% Acrylic acid content, ≤0.005% Water insolubles

vapor density

2.45 (vs air)

vapor density

2.45 (vs air)

vapor density

2.45 (vs air)

vapor density

2.45 (vs air)

vapor pressure

0.03 mmHg ( 40 °C)

vapor pressure

0.03 mmHg ( 40 °C)

vapor pressure

0.03 mmHg ( 40 °C)

vapor pressure

0.03 mmHg ( 40 °C)

assay

≥99% (HPLC)

assay

≥99%

assay

≥99.0% (GC)

assay

≥99% (HPLC)

General description

Acrylamide functions as a quencher of tryptophan fluorescence. Acrylamide along with bis-acrylamide crosslinks to form polyacrylamide gels, which is used to separate protein and other biomolecules. Bis-acrylamide is essential for crosslinking and acrylamide is required for the polymerization of monomers. Acrylamide alkylates SH groups of cysteine and ε-NH2 lysine residues of proteins during electrophoresis.

Application

Acrylamide has been used in the preparation of polyacrylamide gels.

Compatibility

This substance has been tested against several types of hand protection for CE compliance. See Related Products to find the recommended gloves for handling this product.

suggested gloves for splash protection

Product No.
Description
Pricing

Pictograms

Skull and crossbonesHealth hazard

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

Target Organs

Peripheral nervous system

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

280.4 °F - closed cup

Flash Point(C)

138 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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