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A3678

Ammonium persulfate

Molecular Biology, suitable for electrophoresis, ≥98%

Synonym(s):

AP, APS, Ammonium peroxodisulfate, Ammonium peroxydisulfate, PER

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About This Item

Linear Formula:
(NH4)2S2O8
CAS Number:
Molecular Weight:
228.20
PubChem Substance ID:
eCl@ss:
38030421
UNSPSC Code:
41105319
NACRES:
NB.22
EC Number:
231-786-5
MDL number:
Assay:
≥98%
Form:
powder

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assay

≥98%

form

powder

InChI key

ROOXNKNUYICQNP-UHFFFAOYSA-N

InChI

1S/2H3N.H2O8S2/c;;1-9(2,3)7-8-10(4,5)6/h2*1H3;(H,1,2,3)(H,4,5,6)

grade

Molecular Biology

vapor density

7.9 (vs air)

reaction suitability

reagent type: oxidant

technique(s)

electrophoresis: suitable

anion traces

chloride (Cl-): <10 ppm

cation traces

Fe: <10 ppm, heavy metals (as Pb): <50 ppm

foreign activity

DNAse, none detected, Protease, none detected, RNAse, none detected

Quality Level

SMILES string

N.N.OS(=O)(=O)OOS(O)(=O)=O

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1 of 4

This Item
215589A746009913
assay

≥98%

assay

98%

assay

≥98.0%

assay

≥98.0% (RT)

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200

reaction suitability

reagent type: oxidant

reaction suitability

reagent type: oxidant

reaction suitability

reagent type: oxidant

reaction suitability

-

form

powder

form

crystalline powder

form

powder

form

-

grade

for molecular biology

grade

reagent grade

grade

-

grade

for molecular biology

vapor density

7.9 (vs air)

vapor density

7.9 (vs air)

vapor density

7.9 (vs air)

vapor density

7.9 (vs air)

General description

Ammonium persulfate (APS) is a widely used reagent in biochemistry and molecular biology for the preparation of polyacrylamide gels. APS forms oxygen free radicals in aqueous solution by a base-catalyzed mechanism. The bases, most commonly used as catalysts, are tertiary amines such as TEMED (N,N,N′,N′-tetramethylethylenediamine) or DMAPN (3-dimethylaminopropionitrile). The free radicals will cause the polymerization of acrylamide and bis-acrylamide to form a gel matrix, which can be used for separating macromolecules by size. APS has also been utilized to study protein-protein interactions via photoinitiated crosslinking chemistry.

Application

Catalyst for acrylamide gel polymerization.
Ammonium persulfate has been used for the preparation of polyacrylamide gels and acrylamide hydrogels.

Analysis Note

Tested for use in acrylamide polymerization.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Ox. Sol. 3 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

5.1B - Oxidizing hazardous materials

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


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Relationships among cell morphology, intrinsic cell stiffness and cell-substrate interactions.
Chiang MY et al.
Biomaterials, 34, 9754-9754 (2013)
Maurer HR
Disc Electrophoresis and Related Techniques of Polyacrylamide Gel Electrophoresis (1978)
Chemistry for the analysis of protein-protein interactions: rapid and efficient cross-linking triggered by long wavelength light.
Fancy DA and Kodadek T
Proceedings of the National Academy of Sciences of the USA, 96, 6020-6020 (1999)
Composite three-dimensional woven scaffolds with interpenetrating network hydrogels to create functional synthetic articular cartilage.
Liao IC et al.
Advances in Functional Materials, 23, 5833-5833 (2013)
Development of digestive enzyme activity in larvae of spotted sand bass Paralabrax maculatofasciatus II: Electrophoretic analysis.
Alvarez-Gonzalez CA, et al.
Fish Physiology and Biochemistry, 36, 29-29 (2010)

Articles

Bis-Tris SDS-PAGE gel casting kits with mPAGE® TurboMix technology for rapid, reliable gel casting and protein separation.

Protocols

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

Resumen de la química del gel de poliacrilamida e instrucciones detalladas para preparar a mano geles de poliacrilamida con los kits de moldeo del gel bis-Tris TurboMix mPAGE®

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      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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