A3688

Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

• Synonym(s): Goat Anti-Mouse IgG (whole molecule)−AP
• MDL number: MFCD00162640
• NACRES: NA.46

Properties

• biological source: goat
• Quality Level: 200
• conjugate: alkaline phosphatase conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous glycerol solution
• species reactivity: mouse
• should not react with: human
• technique(s): direct ELISA: 1:30,000; dot blot: 1:30,000; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50; western blot: 1:30,000
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Goat Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody react with normal mouse serum and mouse IgG using IEP. By ODD, the antiserum is reacts with mouse IgG1, IgG2a, IgG2b, IgG3, IgA and IgM. The conjugate shows no reactivity with human serum proteins by ELISA.

Immunogen

purified mouse IgG

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Goat Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody for immunocytochemistry assays and ELISA.

Human and Murine tumor cell lysate were analyzed for various protein expression by western blot using alkaline phosphatase conjugated goat anti-mouse IgG as the secondary for 1 hour at 37 degrees.

Surfactant Protein A was detected in bronchoalveolar fluid using alkaline phosphatase conjugated goat anti-mouse IgG as the secondary at μg/ml in TBS/Tween containing final concentration of 0.5M NaCl.

Other Notes

Antibody adsorbed with human serum proteins.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 10 mM glycine, 1 mM MgCl₂, 50% glycerol and 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background with human samples.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable