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A3699

Sigma-Aldrich

Acrylamide/Bis-acrylamide, 30% solution

BioReagent, suitable for electrophoresis, 37.5:1

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Synonym(s):
30% Acrylamide/Bisacrylamide 37.5:1, Polyacrylamide solution
MDL number:
PubChem Substance ID:
NACRES:
NA.25

sterility

0.2 μm filtered

Quality Level

product line

BioReagent

form

liquid

feed ratio

37.5:1

technique(s)

electrophoresis: suitable

suitability

suitable for electrophoresis

storage temp.

2-8°C
2-8°C

SMILES string

NC(=O)C=C.C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)

InChI key

OCQZXMCGTAWGEQ-UHFFFAOYSA-N

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This Item
A3574A9926A2917
Acrylamide/Bis-acrylamide, 30% solution BioReagent, suitable for electrophoresis, 37.5:1

Sigma-Aldrich

A3699

Acrylamide/Bis-acrylamide, 30% solution

Essential+ Grade
Acrylamide/Bis-acrylamide, 30% solution BioReagent, suitable for electrophoresis, 29:1

Sigma-Aldrich

A3574

Acrylamide/Bis-acrylamide, 30% solution

Essential+ Grade
Acrylamide/Bis-acrylamide, 40% solution BioReagent, suitable for electrophoresis, 19:1

Sigma-Aldrich

A9926

Acrylamide/Bis-acrylamide, 40% solution

Essential+ Grade
Acrylamide/Bis-acrylamide BioReagent, for molecular biology, 19:1 (ratio)

Sigma-Aldrich

A2917

Acrylamide/Bis-acrylamide

Essential+ Grade
feed ratio

37.5:1

feed ratio

29:1

feed ratio

19:1

feed ratio

19:1 (ratio)

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

-

suitability

suitable for electrophoresis

suitability

suitable for electrophoresis

suitability

suitable for electrophoresis

suitability

-

sterility

0.2 μm filtered

sterility

0.2 μm filtered

sterility

0.2 μm filtered

sterility

-

product line

BioReagent

product line

BioReagent

product line

BioReagent

product line

BioReagent

General description

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

Application

Acrylamide/Bis-acrylamide, 30% solution has been used:
  • in the preparation of polyacrylamide gel for photobleaching experiment
  • as a constituent in the preparation of gel precursor solution and pore-gradient microgel precursor solution
  • in the preparation of polyacrylamide gels during western blot analyses

These 30% (w/w) acrylamide/bis-acrylamide solutions are available at the most common ratios for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Preparation Note

Solution prepared from electrophoresis grade acrylamide and bis-acrylamide in ultra-pure water.

Pictograms

Exclamation markHealth hazard

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

Target Organs

Peripheral nervous system

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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