MilliporeSigma
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A4043

Sigma-Aldrich

Azure B

certified by the Biological Stain Commission

Synonym(s):
N,N,N′-Trimethylthionin, Azure I, Methyleneazure
Empirical Formula (Hill Notation):
C15H16ClN3S
CAS Number:
Molecular Weight:
305.83
Colour Index Number:
52010
Beilstein:
3922630
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

form

powder

composition

Dye content, ≥89%

mp

205-210 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL

fluorescence

λex 648 in ethanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

C[N+](C)=C(C=C1)C=C(C1=N2)SC3=C2C=CC(NC)=C3.[Cl-]

InChI

1S/C15H15N3S.ClH/c1-16-10-4-6-12-14(8-10)19-15-9-11(18(2)3)5-7-13(15)17-12;/h4-9H,1-3H3;1H

InChI key

DNDJEIWCTMMZBX-UHFFFAOYSA-N

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This Item
227935A2918861049
Azure B certified by the Biological Stain Commission

Sigma-Aldrich

A4043

Azure B

Azure B prepared by direct synthesis

Sigma-Aldrich

227935

Azure B

Azure A chloride certified by the Biological Stain Commission

Sigma-Aldrich

A2918

Azure A chloride

Azure A chloride certified by the Biological Stain Commission, Dye content 70 %

Sigma-Aldrich

861049

Azure A chloride

form

powder

form

powder

form

powder

form

powder

composition

Dye content, ≥89%

composition

-

composition

Dye content, ≥75%

composition

Dye content, 70%

mp

205-210 °C (dec.) (lit.)

mp

205-210 °C (dec.) (lit.)

mp

290 °C (dec.) (lit.)

mp

290 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL

solubility

water: 1 mg/mL, blue to very deep blue

solubility

water: 1 mg/mL

solubility

H2O: 1 mg/mL

fluorescence

λex 648 in ethanol

fluorescence

-

fluorescence

-

fluorescence

-

Application

Azure B has been used for the staining of chromosomes.

Biochem/physiol Actions

Azure B is a cationic dye. It is part of the Romanowsky stain, which is used for the staining of blood films. It is also used for the localization of nucleic acids.

Suitability

Certified for use in Lillie′s modified Nocht′s method for paraffin sections and the NCCLS method for blood smears.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Acute Tox. 4 Oral

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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