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A4058

Acrylamide solution

40%, suitable for electrophoresis, sterile-filtered

Synonym(s):

2-Propenamide, 40% Acrylamido solution, Acrylic amide, Akrylamid, Polyacrylamide solution

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100 ML

$59.30

5 L

$1,630.00

$59.30

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About This Item

Linear Formula:
CH2=CHCONH2
CAS Number:
79-06-1
Molecular Weight:
71.08
NACRES:
NB.22
PubChem Substance ID:
24890742
eCl@ss:
39031205
UNSPSC Code:
41105319
MDL number:
MFCD00008032
Beilstein/REAXYS Number:
6185892

Key Documents

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InChI key

HRPVXLWXLXDGHG-UHFFFAOYSA-N

InChI

1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)

SMILES string

NC(=O)C=C

sterility

sterile-filtered

form

liquid

concentration

40%

technique(s)

electrophoresis: suitable

storage temp.

2-8°C

Quality Level

200

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This Item
A8887A3553A9099
Acrylamide solution 40%, suitable for electrophoresis, sterile-filtered

Sigma-Aldrich

A4058

Acrylamide solution

Acrylamide suitable for electrophoresis, ≥99%

Sigma-Aldrich

A8887

Acrylamide

Acrylamide suitable for electrophoresis, ≥99% (HPLC), powder

Sigma-Aldrich

A3553

Acrylamide

Acrylamide Molecular Biology, ≥99% (HPLC)

Sigma-Aldrich

A9099

Acrylamide

concentration

40%

concentration

-

concentration

-

concentration

-

form

liquid

form

crystals

form

powder

form

crystals

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

-

sterility

sterile-filtered

sterility

-

sterility

-

sterility

-

storage temp.

2-8°C

storage temp.

-

storage temp.

-

storage temp.

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Acrylamide has been classified as probably carcinogenic in humans. It is formed in fried and oven-cooked foods due to Maillard reaction between amino acids and reducing sugars.[1]

Application

Acrylamide solution is suitable for use in electrophoresis. It may be used in the preparation of gel buffer for the determination of molecular weight of polypeptide chains by polyacrylamide gel electrophoresis.[2] It may be used in the electrochemical polymerization of pyrrole. It may be used to prepare the pH 7.6 gels, required for the determination of molecular weights of histone by sodium dodecyl sulfate gel electrophoresis.[3]

Preparation Note

Prepared from electrophoresis grade acrylamide in ultra-pure water.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

target_organs

Peripheral nervous system

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
0000536540
0000536540
0000480675
0000480675
0000452027

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Electrochemical polymerization of pyrrole in acrylamide solution.
Sarac AS, et al.
Synthetic Metals, 98(3), 177-182 (1999)
Donald S Mottram et al.
Nature, 419(6906), 448-449 (2002-10-09)
Reports of the presence of acrylamide in a range of fried and oven-cooked foods have caused worldwide concern because this compound has been classified as probably carcinogenic in humans. Here we show how acrylamide can be generated from food components
The molecular weights of vertebrate histones exploiting a modified sodium dodecyl sulfate electrophoretic method.
S Panyim et al.
The Journal of biological chemistry, 246(24), 7557-7560 (1971-12-25)
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
K Weber et al.
The Journal of biological chemistry, 244(16), 4406-4412 (1969-08-25)
Arianna Rath et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(39), 15668-15673 (2013-09-11)
SDS/PAGE is universally used in biochemistry, cell biology, and immunology to resolve minute protein amounts readily from tissue and cell extracts. Although molecular weights of water-soluble proteins are reliably determined from their SDS/PAGE mobility, most helical membrane proteins, which comprise
View All Related Papers

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Questions

1–4 of 4 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What is the ratio of acrylamide:bisacrylamide in this product? Is it 37.5:1, 29:1 or 19:1 (or maybe other?)

    1 answer

    1. This product is simply a 40% solution of Acrylamide and does not contain any Bis-acrylamide. Note that there are several Acrylamide/Bis-acrylamide 40% solutions available. Please see the link below to review these product options:
      https://www.sigmaaldrich.com/substance/acrylamidebisacrylamide40solution1234598765

      Helpful?

  4. What is the solvent in this solution. Water? Or organic solvent?

    1 answer

    1. The solution is prepared from electrophoresis grade acrylamide in ultra-pure water and aseptically filtered to insure the highest quality and reproducibility.

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