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A4284

Sigma-Aldrich

L-Asparagine monohydrate

BioReagent, suitable for cell culture

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Synonym(s):
(S)-(+)-2-Aminosuccinamic acid, (S)-2-Aminosuccinic acid 4-amide, L-Aspartic acid 4-amide
Linear Formula:
NH2COCH2CH(NH2)COOH · H2O
CAS Number:
Molecular Weight:
150.13
Beilstein:
1723527
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.75

biological source

synthetic

Quality Level

product line

BioReagent

Assay

≥99% (TLC)

form

powder

technique(s)

cell culture | mammalian: suitable

color

white to off-white

mp

233-235 °C (lit.)

SMILES string

[H]O[H].N[C@@H](CC(N)=O)C(O)=O

InChI

1S/C4H8N2O3.H2O/c5-2(4(8)9)1-3(6)7;/h2H,1,5H2,(H2,6,7)(H,8,9);1H2/t2-;/m0./s1

InChI key

RBMGJIZCEWRQES-DKWTVANSSA-N

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This Item
A7094A8381A4159
L-Asparagine monohydrate BioReagent, suitable for cell culture

Sigma-Aldrich

A4284

L-Asparagine monohydrate

-
L-Asparagine monohydrate from non-animal source, BioReagent, suitable for cell culture, ≥98.0%

Sigma-Aldrich

A7094

L-Asparagine monohydrate

Essential+ Grade
L-Asparagine monohydrate ≥99% (TLC)

Sigma-Aldrich

A8381

L-Asparagine monohydrate

-
L-Asparagine BioReagent, suitable for cell culture, suitable for insect cell culture

Sigma-Aldrich

A4159

L-Asparagine

-
assay

≥99% (TLC)

assay

≥98.0%

assay

≥99% (TLC)

assay

≥98% (HPLC)

form

powder

form

powder

form

powder

form

powder

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable

color

white to off-white

color

white

color

white to off-white

color

white

mp

233-235 °C (lit.)

mp

233-235 °C (lit.)

mp

233-235 °C (lit.)

mp

235 °C (dec.) (lit.)

Application

L-Asparagine monohydrate has been used:
  • as a media supplement to culture Black Mexican Sweet (BMS) cells
  • to prepare Adams III media to supplement C+Y medium for culturing Streptococcus pneumoniae
  • in an asparagine-rich diet to study the E0771 syngeneic breast tumor growth and survival in mice

Biochem/physiol Actions

L-Asparagine (L-Asp) is a nonessential amino acid involved in the synthesis of critical cellular proteins of human cells. It is synthesized from oxaloacetic acid. L-Asp is used as a growth supplement for tumor cells in in vitro conditions.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
Pack Size/Quantity

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705578-5MG-PW

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MMYOMAG-74K-13

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Perform colorimetric assays for nonradioactive quantification of cellular proliferation, viability, and cytotoxicity for adherent or suspension cells cultured in 96-well microplates.

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MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.

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