A4378

Sigma-Aldrich

Bovine Serum Albumin

lyophilized powder, 1× crystallized, ≥97% (agarose gel electrophoresis)

Synonym(s):
Albumin bovine serum, Bovine albumin, BSA
CAS Number:
EC Number:
MDL number:
NACRES:
NA.27

Quality Level

biological source

bovine

assay

≥97% (agarose gel electrophoresis)

form

lyophilized powder

quality

crystallized

mol wt

~66 kDa

purified by

cold ethanol fractionation

packaging

poly bottle of

origin

USA origin

application(s)

ELISA: suitable

pH

7

solubility

water: soluble (50 mg/ml)

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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General description

Albumin is the most abundant plasma protein in humans. High levels of albumin are associated with dehydration. Albumin turnover is seen in infants with iron deficiency anemia. Serum albumin is a reliable prognostic indicator for liver disease. Oxidative damage of albumin is associated with advanced liver disease.

Application

Bovine Serum Albumin has been:
  • as an additive in washout solution and fluorescent dye solution
  • as a supplement in Waymouth medium and modified essential medium-α (MEMα )
  • in plant extract for the protein precipitation test
  • as a component in Ringer′s solution to rinse the retinal tissue

Packaging

1, 5, 10, 25, 100 g in poly bottle

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Often referred to as Cohn fraction V; this product is prepared by a modified method of the Cohn cold ethanol fractionation method. Further purified by a proprietary crystallization method
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Distribution of potassium conductance in mammalian Muller (glial) cells: a comparative study
Newman EA
The Journal of Neuroscience, 7(8), 2423-2432 (1987)
Effects of condensed tannins prepared from leaves of fodder plants on digestive enzymes in vitro and in the intestine of rats
Horigome T, et al.
British Journal of Urology, 60(2), 275-285 (1988)
A revised protocol for in vitro development of mouse oocytes from primordial follicles dramatically improves their developmental competence
O'Brien MJ, et al.
Biology of Reproduction, 68(5), 1682-1686 (2003)
Bingmei M Fu et al.
Microvascular research, 68(1), 51-62 (2004-06-29)
To investigate the effect of vascular endothelial growth factor (VEGF) on solute permeability of mammalian microvessels, we measured the apparent permeability (P) of various-sized solutes on the postcapillary venules of rat mesentery in vivo. Exposure to 1 nM VEGF transiently...
Hanna M Eilken et al.
Nature communications, 8(1), 1574-1574 (2017-11-18)
Pericytes adhere to the abluminal surface of endothelial tubules and are required for the formation of stable vascular networks. Defective endothelial cell-pericyte interactions are frequently observed in diseases characterized by compromised vascular integrity such as diabetic retinopathy. Many functional properties...

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