A4416

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti Mouse Hrp Sigma
MDL number:
NACRES:
NA.46

Quality Level

biological source

goat

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

application(s)

direct ELISA: 1:10,000

conjugate

peroxidase conjugate

Featured Industry

Research Pathology

shipped in

dry ice

storage temp.

−20°C

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General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice. Anti-Mouse IgG (whole molecule)-Peroxidase antibody is specific for mouse IgGs.

Immunogen

Purified mouse IgG

Application

Anti-Mouse IgG (whole molecule)-Peroxidase antibody is suitable for use in immunoblot (1:2000). The product can also be used for direct ELISA: 1:10,000.
Western blot analysis of transfected Hela cell lysates was performed using HRP conjugated goat anti-mouse IgG as the secondary at a dilution of 1:4000.

Packaging

0.5 mL in glass insert
1, 5×1 mL in glass bottle

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative.

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

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The Journal of cell biology, 192(6), 1005-1021 (2011-03-23)
The contractile ring is essential for cytokinesis in most fungal and animal cells. In fission yeast, cytokinesis nodes are precursors of the contractile ring and mark the future cleavage site. However, their assembly and architecture have not been well described....
F F Hsieh et al.
Blood, 96(8), 2746-2754 (2000-10-07)
Progression through the mammalian cell cycle is regulated by cyclins, cyclin- dependent kinases (CDKs), and cyclin-dependent kinase inhibitors (CKIs). The function of these proteins in the irreversible growth arrest associated with terminally differentiated cells is largely unknown. The function of...
Lorenzo K Love et al.
Journal of applied physiology (Bethesda, Md. : 1985), 111(2), 427-434 (2011-05-21)
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Eukaryotic cell, 7(9), 1582-1590 (2008-07-16)
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