All Photos(3)

A4550

Sigma-Aldrich

Agar

Type A, suitable for plant cell culture

Synonym(s):
Agar-agar, Gum agar
Linear Formula:
(C12H18O9)n
CAS Number:
EC Number:
MDL number:
eCl@ss:
42040102
NACRES:
NA.21

Quality Level

biological source

algae (Rhodophyceae)

type

Type A

form

powder

application(s)

cell culture | plant: suitable

transition temp

transition temp 32-38 °C (1.5%)

gel strength

>650 g/cm2

Featured Industry

Agriculture

storage temp.

room temp

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General description

Agar (Type A) is extracted from agarocytes of the red alga Rhodophyceae. It is a polysaccharide complex, consisting of a neutral gelling fraction, agarose, and a non-gelling fraction, agaropectin. Type-A agar forms a thermoreversible gel, and is most suitable for plant cell culture.

Application

Type A agar has been used:
  • to perform metal(loid)-related phenotyping of the Arabidopsis thaliana metal-sensitive mutant strains
  • as a component of Murashige and Skoog (MS) media to culturing Arabidopsis thaliana seeds
  • to culture Cicer arietinum L. (Chickpea) seeds for Agrobacterium rhizogenes mediated root transformation

Packaging

500 g in poly bottle
1 kg in poly bottle
5 kg in poly drum

Storage Class Code

11 - Combustible Solids

WGK Germany

WGK 1

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Antoine Desrut et al.
Journal of experimental botany, 71(22), 7301-7315 (2020-08-30)
Plants live in close relationships with complex populations of microorganisms, including rhizobacterial species commonly referred to as plant growth-promoting rhizobacteria (PGPR). PGPR are able to improve plant productivity, but the molecular mechanisms involved in this process remain largely unknown. Using...
Extraction and characterization of agar from Australian Pterocladia lucida
Chiovitti A, et al.,
Journal of Applied Phycology, 16(1), 41-48 (2004)
An SMU Splicing Factor Complex Within Nuclear Speckles Contributes to Magnesium Homeostasis in Arabidopsis.
Feng, et al.
Plant Physiology, 184, 428-442 (2020)
Malika Affaf Dahmani et al.
Frontiers in plant science, 11, 124-124 (2020-03-17)
Plants live in association with complex populations of microorganisms, including Plant Growth-Promoting Rhizobacteria (PGPR) that confer to plants an improved growth and enhanced stress tolerance. This large and diverse group includes endophytic bacteria that are able to colonize the internal...
Pooja Rani Aggarwal et al.
Plant methods, 14, 55-55 (2018-07-11)
Chickpea (Cicer arietinum L.), an important legume crop is one of the major source of dietary protein. Developing an efficient and reproducible transformation method is imperative to expedite functional genomics studies in this crop. Here, we present an optimized and...

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