A4612

Sigma-Aldrich

Bovine Serum Albumin

lyophilized powder, Essentially fatty acid free

Synonym(s):
Albumin bovine serum, Bovine albumin, Albumin from bovine serum, BSA
CAS Number:
EC Number:
MDL number:
NACRES:
NA.25

Quality Level

biological source

bovine

assay

≥98% (agarose gel electrophoresis)

form

lyophilized powder

mol wt

~66 kDa

packaging

poly bottle of

application(s)

ELISA: suitable
cell culture | mammalian: suitable

impurities

≤0.01% free fatty acid (GC)

pH

4.8-7.5

solubility

H2O: soluble 40 mg/mL

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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General description

Bovine serum albumin (BSA) is an α-helical, globular and non-glycosylated 66kDa protein. It is characterized with three domains with two subdomains under each. BSA belongs to the serum albumins family and has 17-disulfide bonds.
The most abundant plasma protein in mammals is albumin. It is produced in the liver and transferred as a non-glycosylated protein into the plasma. This multifunctional protein has an amazing ability to bind to ligands.

Application

Bovine serum albumin (BSA) is extensively used for drug delivery since it is widely available, cheap and easily purified, has ligand-binding properties, and is widely accepted in the pharmaceutical industry.
Bovine Serum Albumin has been used as a component of Tris-buffered saline (TBST) to stabilize polyvinylidene fluoride (PVDF) membranes during western blotting. It has also been used in along with phosphate-buffered saline with tween detergent (PBST) as a blocking buffer in enzyme linked immunosorbent assay(ELISA).

Packaging

1, 25, 100 g in poly bottle
1 kg in poly bottle

Biochem/physiol Actions

Albumin is the most important circulatory protein involved in the control of Ca2+ (and Mg2+) levels in mammals.
Bovine Serum Albumin (BSA) is a transporter for drugs, hormones and fatty acids. It is used as a blocking agent in enzyme linked immunosorbent assay (ELISA) for preventing non-specific binding of antigens and antibodies to the microtiter plates. BSA is a crucial component of the cell culture media and favors embryonic stem cells (hESC) differentiation. BSA shares structural features with human serum albumin. Its high solubility, low cost and purity and interaction with surfactant is exploited in cosmetic and pharmaceutical industry.
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Features and Benefits

  • Essentially fatty acid-free
  • Sourced and manufactured in New Zealand
  • Prepared by chromatography

Preparation Note

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Targeting the PI3K/AKT pathway via GLI1 inhibition enhanced the drug sensitivity of acute myeloid leukemia cells
Liang H, et al.
Scientific reports, 7, 40361-40361 (2017)
Inhibition of the fibrillation of highly amyloidogenic human calcitonin by cucurbit [7] uril with improved bioactivity
Shang H, et al.
Acta Biomaterialia, 78, 178-188 (2018)
Yuangang Zu et al.
International journal of nanomedicine, 8, 1207-1222 (2013-04-10)
The livertaxis of glycyrrhizic acid-conjugated bovine serum albumin (GL-BSA) has been reported in the literature. Now, in this paper, we describe a novel type of drug-targeted delivery system containing 10-hydroxycamptothecin (HCPT) with liver tumor targeting. First, GL was coupled to...
Lin Luo et al.
Journal of leukocyte biology, 107(2), 251-262 (2019-08-31)
In innate immune cells, pathogens and danger signals activate TLRs, unleashing potent and tailored inflammatory responses. Previously, we reported that an immune-specific transmembrane adaptor, SLP adaptor and CSK interacting membrane protein (SCIMP), interacts with TLR4 via direct binding to its...
Heather E Fice et al.
Genes, 10(7) (2019-07-25)
Telomeres are repeat regions of DNA that cap either end of each chromosome, thereby providing stability and protection from the degradation of gene-rich regions. Each cell replication causes the loss of telomeric repeats due to incomplete DNA replication, though it...

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