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A4685

Sigma-Aldrich

Luminol sodium salt

Synonym(s):
3-Aminophthalhydrazide, 5-Amino-2,3-dihydro-1,4-phthalazinedione
Linear Formula:
C8H6N3O2Na
CAS Number:
Molecular Weight:
199.14
MDL number:
PubChem Substance ID:
NACRES:
NA.83

Assay

≥98% (titration)

Quality Level

form

powder and chunks

solubility

water: 50 mg/mL, clear to very slightly hazy, colorless to faint red

SMILES string

O=C1NN([Na])C(C2=CC=CC(N)=C21)=O

InChI

1S/C8H7N3O2.Na/c9-5-3-1-2-4-6(5)8(13)11-10-7(4)12;/h1-3H,(H4,9,10,11,12,13);/q;+1/p-1

InChI key

RVJVDCVIJCBUTH-UHFFFAOYSA-M

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This Item
A8511123072A8264
Luminol sodium salt

Sigma-Aldrich

A4685

Luminol sodium salt

Luminol ≥97% (HPLC)

Sigma-Aldrich

A8511

Luminol

Luminol 97%

Sigma-Aldrich

123072

Luminol

4-Aminophthalhydrazide

Sigma-Aldrich

A8264

4-Aminophthalhydrazide

form

powder and chunks

form

powder or chunks

form

-

form

powder

solubility

water: 50 mg/mL, clear to very slightly hazy, colorless to faint red

solubility

DMSO: 50 mg/mL, clear to slightly hazy

solubility

-

solubility

2 M NH4OH: 50 mg/mL, clear to slightly hazy

Application

Luminol sodium salt has been used:

  • as a component of MEBSS buffer in a bioluminescence assay to detect peroxidase activity in whole blood samples
  • to study protein interaction in whole-cell lysates by immunoprecipitation
  • for in vitro reactive oxygen species (ROS) quantification in CD45+ and CD45− cells

Biochem/physiol Actions

Luminol is a redox-sensitive chemiluminescent compound. It emits blue luminescence when the basic aqueous solution of luminol having oxygen atoms come in contact with oxidizing agents like potassium ferricyanide. It is highly stable and is applicable in various fields such as analytical chemistry, metallurgy, biochemistry, and forensics for detecting reactive species. Luminol has the potential to detect defects in neutrophils ex vivo during oxidative metabolism defects such as chronic granulomatous disease and myeloperoxidase deficiency.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Opioids prevent regeneration in adult mammals through inhibition of ROS production
Elodie L, et al.
Scientific Reports, 8(1) (2018)
Yantu Zhang et al.
Journal of chromatography. A, 1154(1-2), 260-268 (2007-04-24)
A novel method was developed for the simultaneous determination of glucocorticoid residues such as triamcinolone (TR), prednisolone (PR), hydrocortisone (HC), cortisone (CO), methylprednisolone (MP), dexamethasone (DE) and triamcinolone acetonide (TA) by high-performance liquid chromatography (HPLC) coupled with chemiluminescence (CL) detection.
Chemiluminescence detection in analytical chemistry
Lancaster, J. Steven
Endeavour, 16, 194-194 (1992)
New Developments in Chemiluminescence Research
Gill, Steven, K.
Aldrichimica Acta, 16, 59-59 (1983)
Luminol Chemiluminescence-Delay Method for Determination of Copper(II)
Kamidate, Tamio; Ishikawa, Atsushi; Watanabe, Hiroto.
Bulletin of the Chemical Society of Japan, 65, 1591-1591 (1992)

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