A4914

Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat

Name: Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat
Brand: SIGMA
Price: 217 USD

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Goat Anti-Rabbit IgG (whole molecule)–HRP

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1 ML

$217.00

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About This Item

MDL number:

MFCD00162788

UNSPSC Code:

12352203

NACRES:

NA.46

Key Documents

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biological source

goat

Quality Level

200

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:10,000

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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Related Categories

Antibodies - Primary, Secondary & Recombinant Monoclonal

Protein Biology

Secondary Antibodies

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Show Differences

1 of 4

This Item	A8275	A5420	A8924
Sigma-Aldrich A4914 Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat	$Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich A8275 Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat	Sigma-Aldrich A5420 Anti-Goat IgG (whole molecule)–Peroxidase antibody produced in rabbit	Sigma-Aldrich A8924 Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in goat
conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate peroxidase conjugate
technique(s) direct ELISA: 1:10,000	technique(s) direct ELISA: 1:10,000	technique(s) direct ELISA: 1:40,000, dot blot: 1:80,000-1:160,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200	technique(s) direct ELISA: 1:10,000
biological source goat	biological source goat	biological source rabbit	biological source goat
antibody form affinity isolated antibody	antibody form IgG fraction of antiserum	antibody form affinity isolated antibody	antibody form affinity isolated antibody
form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution
clone polyclonal	clone polyclonal	clone polyclonal	clone polyclonal

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit polyclonal IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary polyclonal anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the immunological analysis of target proteins. Anti-Rabbit IgG (whole molecule)-Peroxidase antibody is specific for all rabbit IgGs and does not bind to human serum proteins in ELISA.

Immunogen

Purified rabbit IgG

Application

Anti-Rabbit IgG (whole molecule)-Peroxidase antibody can be used in sandwich ELISA (diluted 1:3000 in PBS-0.05% Tween 20) and immunoelectrophoresis. The product can also be used for western blotting applications (at 1:2000 dilutions in mouse liver homogenates , or in mouse embryonic fibroblasts ).

Co-immunoprecipation and western blot analysis of C33A cell lysates were performed using HRP conjugated goat anti-rabbit IgG as the secondary antibody.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative.

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Antibody adsorbed with human serum proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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pictograms

GHS07,GHS09

signalword

Warning

hcodes

H315,H317,H319,H411

pcodes

P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0000502284

0000486857

0000465585

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Inflammasome activation by Campylobacter jejuni.

Lieneke I Bouwman et al.

Journal of immunology (Baltimore, Md. : 1950), 193(9), 4548-4557 (2014-10-01)

The Gram-negative pathogen Campylobacter jejuni is the most common cause of bacterial foodborne disease worldwide. The mechanisms that lead to bacterial invasion of eukaryotic cells and massive intestinal inflammation are still unknown. In this study, we report that C. jejuni

Allopregnanolone alters follicular and luteal dynamics during the estrous cycle.

Joana Antonela Asensio et al.

Reproductive biology and endocrinology : RB&E, 16(1), 35-35 (2018-04-11)

Allopregnanolone is a neurosteroid synthesized in the central nervous system independently of steroidogenic glands; it influences sexual behavior and anxiety. The aim of this work is to evaluate the indirect effect of a single pharmacological dose of allopregnanolone on important

Effects of slow-release urea and rumen-protected methionine and histidine on mammalian target of rapamycin (mTOR) signaling and ubiquitin proteasome-related gene expression in skeletal muscle of dairy cows.

H Sadri et al.

Journal of dairy science, 99(8), 6702-6713 (2016-05-18)

The mammalian target of rapamycin (mTOR) is a major regulator of protein synthesis, whereas the ubiquitin-proteasome system (UPS) is regarded as the main proteolytic pathway in skeletal muscle. The objective of the current study was to investigate the effects of

Using primary murine intestinal enteroids to study dietary TAG absorption, lipoprotein synthesis, and the role of apoC-III in the intestine.

Javeed Jattan et al.

Journal of lipid research, 58(5), 853-865 (2017-02-06)

Since its initial report in 2009, the intestinal enteroid culture system has been a powerful tool used to study stem cell biology and development in the gastrointestinal tract. However, a major question is whether enteroids retain intestinal function and physiology.

Cell-free translation and purification of Arabidopsis thaliana regulator of G signaling 1 protein.

Bo Li et al.

Protein expression and purification, 126, 33-41 (2016-05-11)

Arabidopsis thaliana Regulator of G protein Signalling 1 (AtRGS1) is a protein with a predicted N-terminal 7-transmembrane (7TM) domain and a C-terminal cytosolic RGS1 box domain. The RGS1 box domain exerts GTPase activation (GAP) activity on Gα (AtGPA1), a component

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